It is well known that the plasminogen-activating (PA) system plays a key role in the bovine mammary gland during tissue remodelling. However, the modulation of the PA cascade after bacterial infections needs to be elucidated. This study examined the effects of Escherichia coli lipopolysaccharide (LPS) on cell viability, the modulation of cell-associated u-PA activity, and the regulation of u-PA and u-PA receptor (uPAR) RNA expression using the BME-UV1 bovine mammary epithelial cell line. LPS did not affect cell viability, but induced an increase in u-PA activity, with the maximum response after 6 h of incubation. Moreover, u-PA and u-PAR mRNA expression were both up-regulated in BME-UV1 cells after 3 h of incubation with LPS. These data indicated that E. coli LPS led to an increase in u-PA activity and RNA expression of u-PA and u-PAR in BME-UV1 cells, thus strengthening the role of the PA system during pathological processes.
Effect of Escherichia coli lipopolysaccharide on u-PA activity and u-PA and u-PAR RNA expression in a bovine mammary epithelial cell line / A. Baldi, C. Pecorini, R. Rebucci, F. Saccone, F. Cheli, A. Miranda Ribera, C. Lecchi, F. Ceciliani. - In: RESEARCH IN VETERINARY SCIENCE. - ISSN 0034-5288. - 93:2(2012 Oct), pp. 758-762.
|Titolo:||Effect of Escherichia coli lipopolysaccharide on u-PA activity and u-PA and u-PAR RNA expression in a bovine mammary epithelial cell line|
BALDI, ANTONELLA (Primo)
PECORINI, CHIARA (Secondo) (Corresponding)
LECCHI, CRISTINA (Penultimo)
CECILIANI, FABRIZIO (Ultimo)
|Parole Chiave:||Plasminogen-activating system; BME-UV1; Lipopolysaccharide|
|Settore Scientifico Disciplinare:||Settore AGR/18 - Nutrizione e Alimentazione Animale|
Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria
|Data di pubblicazione:||ott-2012|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.rvsc.2011.10.017|
|Appare nelle tipologie:||01 - Articolo su periodico|