The capability of PCR-TTGE to detect meat species in mixed animal samples was investigated as a necessary step in developing a method where the identification will be performed matching on the "DNA barcode" zone the sequences of resolved PCR products obtained from a limited set of "universal" primers. Exemplary mixtures from five important meat species were analyzed. At this stage more PCR reactions have to be applied on a sample but this should be easily improved using primers simultaneously (as a "cocktail") in a single reaction.

Polymerase chain reaction products (PCR) on DNA barcode zone resolved by temporal temperature gradient electophoresis: a tool for species identification of mixed meat specimens : a technical note on preliminary results / F. Colombo, S. Chessa, P. Cattaneo, C.E.M. Bernardi. - In: FOOD CONTROL. - ISSN 0956-7135. - 22:8(2011), pp. 1471-1472.

Polymerase chain reaction products (PCR) on DNA barcode zone resolved by temporal temperature gradient electophoresis: a tool for species identification of mixed meat specimens : a technical note on preliminary results

F. Colombo
Primo
;
S. Chessa
Secondo
;
P. Cattaneo
Penultimo
;
C.E.M. Bernardi
Ultimo
2011

Abstract

The capability of PCR-TTGE to detect meat species in mixed animal samples was investigated as a necessary step in developing a method where the identification will be performed matching on the "DNA barcode" zone the sequences of resolved PCR products obtained from a limited set of "universal" primers. Exemplary mixtures from five important meat species were analyzed. At this stage more PCR reactions have to be applied on a sample but this should be easily improved using primers simultaneously (as a "cocktail") in a single reaction.
Meat species identification; Polymerase chain reaction; Temporal temperature gradient electrophoresis; DNA barcoding
Settore VET/04 - Ispezione degli Alimenti di Origine Animale
2011
Article (author)
File in questo prodotto:
File Dimensione Formato  
Bernardi.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 135.21 kB
Formato Adobe PDF
135.21 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/158224
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 7
  • ???jsp.display-item.citation.isi??? 6
social impact