Since chronic inflammation is involved in the pathogenesis and/or in the degeneration of many diseases, such as obesity, cancer, cardiovascular and neurodegenerative disorders, the study of the anti-inflammatory activity of bioactive compounds is gaining great interest. In this scenario, pigmented potatoes could represent a source of different phytonutrients known for their potential health-promoting activity, including phenolic acids, carotenoids and anthocyanins (ACNs). Among these, ACNs are known to exert beneficial properties against inflammation-related diseases, such as neuroinflammation and obesity, while carotenoid consumption inversely correlates with the incidence of many chronic diseases. Thus, we aimed to study the anti-inflammatory properties of three different upland potato varieties enriched in chlorogenic acid (CGA) (yellow-skinned and white-fleshed Kennebec), carotenoids (red-skinned and yellow-fleshed Desiree) or ACNs (purple skinned and fleshed Bleuet) in THP-1 derived macrophages. The phytonutrient composition of tubers extracts has been characterized by HPLC-DAD and spectrophotometric analysis allowing us to consider Kennebec, Desiree and Bleuet extracts as CGA-rich, carotenoid-rich and ACN-rich respectively. Their anti-inflammatory effect was tested on THP-1 macrophages pre-treated with the three extracts and then challenged with LPS. The dose-dependent effects on gene expression and/or protein levels of pro-inflammatory factors, such as cytokines and other mediators, were evaluated. While at higher doses the three extracts exhibit nearly comparable anti-inflammatory properties, our results show that, when provided at plasmatic concentrations, only the carotenoid- and the ACN-rich varieties are able to counteract LPS-induced inflammation. Our findings suggest that the consumption of the pigmented potatoes enriched in carotenoids or ACNs, such as Desiree and Bleuet, may represent a strategy to prevent chronic inflammation.

Anti-inflammatory potential of pigmented potatoes enriched in chlorogenic acid, carotenoids or anthocyanins / M. Toccaceli, A. Marinelli, L. Bassolino, D. Pacifico, B. Parisi, F. Nicoletti, R. Lo Scalzo, G. Mandolino, K. Petroni. - In: POLYPHENOLS COMMUNICATIONS. - ISSN 2957-4056. - 2:(2023 Jul), pp. P.2.07.101-P.2.07.101. (Intervento presentato al 31. convegno International Conference on Polyphenols tenutosi a Nantes nel 2023).

Anti-inflammatory potential of pigmented potatoes enriched in chlorogenic acid, carotenoids or anthocyanins

M. Toccaceli
Primo
;
A. Marinelli;K. Petroni
Ultimo
2023

Abstract

Since chronic inflammation is involved in the pathogenesis and/or in the degeneration of many diseases, such as obesity, cancer, cardiovascular and neurodegenerative disorders, the study of the anti-inflammatory activity of bioactive compounds is gaining great interest. In this scenario, pigmented potatoes could represent a source of different phytonutrients known for their potential health-promoting activity, including phenolic acids, carotenoids and anthocyanins (ACNs). Among these, ACNs are known to exert beneficial properties against inflammation-related diseases, such as neuroinflammation and obesity, while carotenoid consumption inversely correlates with the incidence of many chronic diseases. Thus, we aimed to study the anti-inflammatory properties of three different upland potato varieties enriched in chlorogenic acid (CGA) (yellow-skinned and white-fleshed Kennebec), carotenoids (red-skinned and yellow-fleshed Desiree) or ACNs (purple skinned and fleshed Bleuet) in THP-1 derived macrophages. The phytonutrient composition of tubers extracts has been characterized by HPLC-DAD and spectrophotometric analysis allowing us to consider Kennebec, Desiree and Bleuet extracts as CGA-rich, carotenoid-rich and ACN-rich respectively. Their anti-inflammatory effect was tested on THP-1 macrophages pre-treated with the three extracts and then challenged with LPS. The dose-dependent effects on gene expression and/or protein levels of pro-inflammatory factors, such as cytokines and other mediators, were evaluated. While at higher doses the three extracts exhibit nearly comparable anti-inflammatory properties, our results show that, when provided at plasmatic concentrations, only the carotenoid- and the ACN-rich varieties are able to counteract LPS-induced inflammation. Our findings suggest that the consumption of the pigmented potatoes enriched in carotenoids or ACNs, such as Desiree and Bleuet, may represent a strategy to prevent chronic inflammation.
Settore BIO/11 - Biologia Molecolare
lug-2023
Groupe Polyphénols
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/1031173
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