The large consumption of freshwater in fermentations and bio-transformations is a matter of concern for the sustainability of many bio-processes. The use of seawater to perform bio-processes is a sustainable alternative. In this work, we used marine yeasts from deep-sub-seafloor sediments grown in seawater as bio-catalysts to perform the stereoselective reduction of different ketones, and the bio-transformations were accomplished in seawater as well. Strains of Meyerozyma guilliermondii and Rhodotorula mucilaginosa were able to reduce different aromatic ketones with high molar conversions and moderate-to-high enantioselectivity with no significant differences between bio-catalysis performed in seawater and freshwater. Finally, the selected marine yeasts were used for the reduction of key intermediates in seawater for the synthesis of molecules of pharmaceutical interest (desogestrel, norgestrel, gestodene, pramipexole).

Seawater-Based Biocatalytic Strategy : Stereoselective Reductions of Ketones with Marine Yeasts / I. Serra, B. Guidi, G. Burgaud, M.L. Contente, P. Ferraboschi, A. Pinto, C. Compagno, F. Molinari, D. Romano. - In: CHEMCATCHEM. - ISSN 1867-3880. - 8:20(2016 Oct 20), pp. 3254-3260. [10.1002/cctc.201600947]

Seawater-Based Biocatalytic Strategy : Stereoselective Reductions of Ketones with Marine Yeasts

I. Serra
Primo
;
B. Guidi
Secondo
;
M.L. Contente;P. Ferraboschi;A. Pinto
;
C. Compagno;F. Molinari
Penultimo
;
D. Romano
2016

Abstract

The large consumption of freshwater in fermentations and bio-transformations is a matter of concern for the sustainability of many bio-processes. The use of seawater to perform bio-processes is a sustainable alternative. In this work, we used marine yeasts from deep-sub-seafloor sediments grown in seawater as bio-catalysts to perform the stereoselective reduction of different ketones, and the bio-transformations were accomplished in seawater as well. Strains of Meyerozyma guilliermondii and Rhodotorula mucilaginosa were able to reduce different aromatic ketones with high molar conversions and moderate-to-high enantioselectivity with no significant differences between bio-catalysis performed in seawater and freshwater. Finally, the selected marine yeasts were used for the reduction of key intermediates in seawater for the synthesis of molecules of pharmaceutical interest (desogestrel, norgestrel, gestodene, pramipexole).
biocatalysis; ketones; enzymes; reduction; stereoselectivity
Settore CHIM/11 - Chimica e Biotecnologia delle Fermentazioni
   Marine Microorganisms: Cultivation Methods for Improving their Biotechnological Applications
   MACUMBA
   EUROPEAN COMMISSION
   FP7
   311975
20-ott-2016
2016
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/444293
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