Centrate is a low-cost alternative to synthetic fertilizers for microalgal cultivation, reducing environmental burdens and remediation costs. Adapted microalgae need to be selected and characterised to maximise biomass production and depuration efficiency. Here, the performance and composition of six microalgal communities cultivated both on synthetic media and centrate within semi-open tubular photobioreactors were investigated through Illumina sequencing. Biomass grown on centrate, exposed to a high concentration of ammonium, showed a higher quantity of nitrogen (5.6% dry weight) than the biomass grown on the synthetic media nitrate (3.9% dry weight). Eukaryotic inocula were replaced by other microalgae while cyanobacterial inocula were maintained. Communities were generally similar for the same inoculum between media, however, inoculation with cyanobacteria led to variability within the eukaryotic community. Where communities differed, centrate resulted in a higher richness and diversity. The higher nitrogen of centrate possibly led to higher abundance of genes coding for N metabolism enzymes.

Centrate as a sustainable growth medium : impact on microalgal inocula and bacterial communities in tubular photobioreactor cultivation systems / E. Clagnan, G. D'Imporzano, M. Dell'Orto, A. Bani, A.J. Dumbrell, K. Parati, F.G. Acién-Fernández, A. Portillo-Hahnefeld, A. Martel-Quintana, J.L. Gómez-Pinchetti, F. Adani. - In: BIORESOURCE TECHNOLOGY. - ISSN 0960-8524. - 363:(2022 Nov), pp. 127979.1-127979.10. [10.1016/j.biortech.2022.127979]

Centrate as a sustainable growth medium : impact on microalgal inocula and bacterial communities in tubular photobioreactor cultivation systems

E. Clagnan;G. D'Imporzano
;
M. Dell'Orto;A. Bani;F. Adani
Ultimo
2022

Abstract

Centrate is a low-cost alternative to synthetic fertilizers for microalgal cultivation, reducing environmental burdens and remediation costs. Adapted microalgae need to be selected and characterised to maximise biomass production and depuration efficiency. Here, the performance and composition of six microalgal communities cultivated both on synthetic media and centrate within semi-open tubular photobioreactors were investigated through Illumina sequencing. Biomass grown on centrate, exposed to a high concentration of ammonium, showed a higher quantity of nitrogen (5.6% dry weight) than the biomass grown on the synthetic media nitrate (3.9% dry weight). Eukaryotic inocula were replaced by other microalgae while cyanobacterial inocula were maintained. Communities were generally similar for the same inoculum between media, however, inoculation with cyanobacteria led to variability within the eukaryotic community. Where communities differed, centrate resulted in a higher richness and diversity. The higher nitrogen of centrate possibly led to higher abundance of genes coding for N metabolism enzymes.
centrate; microalgae; next generation sequencing, bacteria; vertical bubble-column photobioreactor; waste management
Settore AGR/13 - Chimica Agraria
   Sustainable Algae Biorefinery for Agriculture aNd Aquaculture
   SABANA
   EUROPEAN COMMISSION
   H2020
   727874
nov-2022
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/938339
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