Effects of cell line proliferation on the aggregation and stability of a hyaluronic acid solution (HA)/PLGA microparticles dispersed in the culture system

Increasing efforts have been focused on development of novel therapeutic approaches based on a carrier mixed with polymeric microparticles (MPs) for promoting bioactive factors delivery. Polymers as poly (lactide-co-glycolide) are the most widely used due to their tunable physico-chemical properties and biomimetic character. Here, PLGA-MPs of different surface charges were dispersed in hyaluronic acid and their impact on the viability of four cell lines was studied. Our data reveal that the most evident effect is the influence of the cell line proliferation-rate on PLGA-MPs stability and aggregation, highlighting the crucial role of the biological context in affecting microparticles clustering and toxicity.