Acting through nicotinic acetylcholine receptors (nAChRs), acetylcholine plays an important role in retinal development and the formation of retinal connections to target tissues, but very little is known about the nAChR subtypes expressed in vertebrate retina during neuronal development. We used immunoprecipitation and [3H]epibatidine binding to study the expression of chick retina α-bungarotoxin-insensitive heteromeric nAChRs during development and adulthood, and found that it is strictly developmentally regulated, reaching a peak on postnatal day 1. The increase in [3H]epibatidine receptors is caused mainly by an increase in the receptors containing the α2, α6, β3, and β4 subunits. The contribution of β subunits to [3H]epibatidine receptors significantly changes during development: the β2 subunit is contained in the majority (84%) of receptors on embryonic day (E) 7 but in only 32% on postnatal day (P) 1, whereas the β4-containing receptors increase from 22% to 78% during the same period. Using a sequential immunodepletion procedure, we purified the β2- and β4-containing subtypes and found that they coassemble with α4 and/or α3 on E11, and also with the α2, α6, and α3 on P1. After the immunodepletion of α6-containing receptors, the β2- and β4-containing receptors have a very similar pharmacological profile on P1. Parallel immunoprecipitation experiments in other brain areas showed that the developmentally regulated receptors in optic lobe are those containing the α2, α5, and β2 subunits and those containing the α4 and β2 subunits, whereas the receptors in forebrain-cerebellum contain the α4 and β2 subunits with or without the α5 subunit. These results indicate that there is an increase in receptor heterogeneity and complexity in chick retina during development that is also maintained in adulthood.
|Titolo:||Developmental expression of heteromericnicotinic receptor subtypes in chick retina|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||2003|
|Digital Object Identifier (DOI):||10.1124/mol.63.6.1329|
|Appare nelle tipologie:||01 - Articolo su periodico|