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IRIS è il sistema di gestione integrata dei dati della ricerca (persone, progetti, pubblicazioni, attività) adottato dall'Università degli Studi di Milano.
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EnglishBreast cancer (BC) is a serious and widespread disease for which different treatments have been developed. In addition to the classic therapies, the treatment with retinoic acid (RA) is still being clinically investigated. RA reduces cancer cells proliferation and migration, but its molecular mechanism of action is not clear. In tumor development, autophagy promotes cancer cell survival and prevents apoptosis. Small heat shock protein B8 (HSPB8) acts together with its co-chaperone BCL-2 associated athanogene 3 (BAG3) stimulating BC proliferation and migration. We analyzed whether direct correlations exist between RA and HSPB8 or BAG3 and how this may play a role in BC. We measured HSPB8 and BAG3 gene expression in MCF-7 BC cells and we analyzed the potential correlation between the antiproliferative and antimigratory effect of RA with the expression level of HSPB8. We found that in MCF-7 cells RA reduces both HSPB8 and BAG3 gene expression and it alters the mitotic spindle organization. Notably, the effects of RA on HSPB8 levels are exerted both at transcriptional and translational levels acting also on HSPB8 mRNA levels. RA effects are possibly mediated by miR-574-5p that targets the HSPB8 transcript. Our results suggest that therapeutic doses of RA can efficiently counteract the adverse effects of HSPB8 in BC progression.
Retinoic Acid Downregulates HSPB8 Gene Expression in Human Breast Cancer Cells MCF-7 / M. Piccolella, R. Cristofani, B. Tedesco, M. Chierichetti, V. Ferrari, E. Casarotto, M. Cozzi, V. Crippa, P. Rusmini, M. Galbiati, A. Poletti, E. Messi. - In: FRONTIERS IN ONCOLOGY. - ISSN 2234-943X. - 11(2021), pp. 652085.1-652085.12. [10.3389/fonc.2021.652085]
| Titolo: | Retinoic Acid Downregulates HSPB8 Gene Expression in Human Breast Cancer Cells MCF-7 | |
| Autori: | PICCOLELLA, MARGHERITA (Co-primo) CRISTOFANI, RICCARDO MARIA (Co-primo) MESSI, ELIO (Ultimo) | |
| Parole Chiave: | retinoic acid; HSPB8; breast cancer; miRNAs; proliferation | |
| Settore Scientifico Disciplinare: | Settore BIO/13 - Biologia Applicata Settore BIO/09 - Fisiologia | |
| Progetto: | Alternative translation initiation as a novel strategy to block toxicity of the mutant Androgen Receptor in SBMA Motor neuron degeneration in Spinal and Bulbar Muscular Atrophy: molecular approaches to counteract mutant androgen receptor neurotoxicity Translating molecular mechanisms into ALS risk and patient's well-being Selective translation of androgen receptor isoform A to prevent polyQ mediated toxicity in Kennedy’s disease Colchicine for Amyotrophic Lateral Sclerosis: a phase II, randomized, double blind, placebo controlled, multicenter clinical trial (Co-ALS) The interplay between the “rna/protein quality control system” and “exosomes” as a spreading mechanism in amyotrophic lateral sclerosis (EX_ALS) The involvement of the small heat shock protein HSPB8 in amyotrophic lateral sclerosis Extracellular vescicles in the pathogenesis of frontotemporal dementia RAN translation of normal and expanded nucleotide repeat containing transcripts to neurotoxic polypetides in neurodegenerative diseases Targeting RAN translation in ALS (Target-RAN) | |
| Data di pubblicazione: | 2021 | |
| Rivista: | ||
| Tipologia: | Article (author) | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.3389/fonc.2021.652085 | |
| Appare nelle tipologie: | 01 - Articolo su periodico |
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| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| Author's Proof.pdf | Pre-print (manoscritto inviato all'editore) | Open Access Visualizza/Apri | ||
| fonc-11-652085.pdf | Publisher's version/PDF | Open Access Visualizza/Apri |
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