Enzyme-linked immunosorbent assay (ELISA) is a quantitative analytical method used to measure the concentration of molecules in biological fluids through antigen-antibody reactions. Here we describe the measurement of anti-C1-inhibitorautoantibodies by an indirect ELISA. In this method patients' sera are incubated in a microplate coated with plasma derived C1-inhibitor.
Anti-C1-Inhibitor Autoantibody Detection by ELISA / C. Suffritti, S. Caccia, S. Berra, D. Parolin, M. Cicardi (METHODS IN MOLECULAR BIOLOGY). - In: The Complement System : Innovative Diagnostic and Research Protocols / [a cura di] L.T. Roumenina. - [s.l] : Springer Nature, 2021. - ISBN 9781071610152. - pp. 115-120 [10.1007/978-1-0716-1016-9_11]
Anti-C1-Inhibitor Autoantibody Detection by ELISA
C. SuffrittiPrimo
;S. CacciaSecondo
;S. Berra;D. Parolin;M. CicardiUltimo
2021
Abstract
Enzyme-linked immunosorbent assay (ELISA) is a quantitative analytical method used to measure the concentration of molecules in biological fluids through antigen-antibody reactions. Here we describe the measurement of anti-C1-inhibitorautoantibodies by an indirect ELISA. In this method patients' sera are incubated in a microplate coated with plasma derived C1-inhibitor.
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