The human parasitic disease Schistosomiasis is caused by the Schistosoma trematode flatworm that infects freshwaters in tropical regions of the world, particularly in Sub-Saharan Africa, South America, and the Far-East. It has also been observed as an emerging disease in Europe, due to increased immigration. In addition to improved therapeutic strategies, it is imperative to develop novel, rapid, and sensitive diagnostic tests that can detect the Schistosoma parasite, allowing timely treatment. Present diagnosis is difficult and involves microscopy-based detection of Schistosoma eggs in the feces. In this context, we present the 3.22 Å resolution crystal structure of the circulating antigen Serine protease inhibitor from S. mansoni (SmSPI), and we describe it as a potential serodiagnostic marker. Moreover, we identify three potential immunoreactive epitopes using in silico-based epitope mapping methods. Here, we confirm effective immune sera reactivity of the recombinant antigen, suggesting the further investigation of the protein and/or its predicted epitopes as serodiagnostic Schistosomiasis biomarkers.

Structure, Immunoreactivity, and In Silico Epitope Determination of {SmSPI} S. mansoni Serpin for Immunodiagnostic Application / S. De Benedetti, F. Di Pisa, E.M.A. Fassi, M. Cretich, A. Music(`(o)), R. Frigerio, A. Mussida, M. Bombaci, R. Grifantini, G. Colombo, M. Bolognesi, R. Grande, N. Zanchetta, M.R. Gismondo, D. Mileto, A. Mancon, L.J. Gourlay. - In: VACCINES. - ISSN 2076-393X. - 9:4(2021 Apr 01), pp. 322.1-322.12. [10.3390/vaccines9040322]

Structure, Immunoreactivity, and In Silico Epitope Determination of {SmSPI} S. mansoni Serpin for Immunodiagnostic Application

S. De Benedetti
Primo
;
F. Di Pisa
Secondo
;
E.M.A. Fassi;M. Bolognesi;M.R. Gismondo;D. Mileto;A. Mancon
Penultimo
;
L.J. Gourlay
Ultimo
2021

Abstract

The human parasitic disease Schistosomiasis is caused by the Schistosoma trematode flatworm that infects freshwaters in tropical regions of the world, particularly in Sub-Saharan Africa, South America, and the Far-East. It has also been observed as an emerging disease in Europe, due to increased immigration. In addition to improved therapeutic strategies, it is imperative to develop novel, rapid, and sensitive diagnostic tests that can detect the Schistosoma parasite, allowing timely treatment. Present diagnosis is difficult and involves microscopy-based detection of Schistosoma eggs in the feces. In this context, we present the 3.22 Å resolution crystal structure of the circulating antigen Serine protease inhibitor from S. mansoni (SmSPI), and we describe it as a potential serodiagnostic marker. Moreover, we identify three potential immunoreactive epitopes using in silico-based epitope mapping methods. Here, we confirm effective immune sera reactivity of the recombinant antigen, suggesting the further investigation of the protein and/or its predicted epitopes as serodiagnostic Schistosomiasis biomarkers.
Neglected Tropical Disease; circulating antigen; crystal structure; in silico epitope predictions; schistosomiasis; Serine protease inhibitor; immunodiagnostics;
Settore BIO/10 - Biochimica
Network Regionale per lo Sviluppo di metodi Diagnostici in Risposta rapida a epidemie emergenti e bioemergenze (READy)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/830647
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