The development of 3D printable hydrogels based on the crosslinking between chitosan and gelatin is proposed. Chitosan and gelatin were both functionalized with methyl furan groups. Chemical modification was performed by reductive amination with methyl furfural involving the lysine residues of gelatin and the amino groups of chitosan to generate hydrogels with tailored properties. The methyl furan residues present in both polymers were exploited for efficient crosslinking via Diels-Alder ligation with PEG-Star-maleimide under cell-compatible conditions. The obtained chitosan-gelatin hybrid was employed to formulate hydrogels and 3D printable biopolymers and its processability and biocompatibility were preliminarily investigated.

Design and Synthesis of Chitosan—Gelatin Hybrid Hydrogels for 3D Printable in vitro Models / S. Magli, G.B. Rossi, G. Risi, S. Bertini, C. Cosentino, L. Crippa, E. Ballarini, G. Cavaletti, L. Piazza, E. Masseroni, F. Nicotra, L. Russo. - In: FRONTIERS IN CHEMISTRY. - ISSN 2296-2646. - 8(2020 Jul 14), pp. 524.1-524.13. [10.3389/fchem.2020.00524]

Design and Synthesis of Chitosan—Gelatin Hybrid Hydrogels for 3D Printable in vitro Models

L. Piazza;E. Masseroni;
2020-07-14

Abstract

The development of 3D printable hydrogels based on the crosslinking between chitosan and gelatin is proposed. Chitosan and gelatin were both functionalized with methyl furan groups. Chemical modification was performed by reductive amination with methyl furfural involving the lysine residues of gelatin and the amino groups of chitosan to generate hydrogels with tailored properties. The methyl furan residues present in both polymers were exploited for efficient crosslinking via Diels-Alder ligation with PEG-Star-maleimide under cell-compatible conditions. The obtained chitosan-gelatin hybrid was employed to formulate hydrogels and 3D printable biopolymers and its processability and biocompatibility were preliminarily investigated.
3D bioprinting; 3D cultures; click chemistry for 3D cellular models; Diels-Alder click reaction; functionalization strategies; glycopolymers; hybrid hydrogels
Settore CHIM/06 - Chimica Organica
Settore AGR/15 - Scienze e Tecnologie Alimentari
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/809239
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