Coagulase-negative staphylococci (CNS) are the most prevalent microorganisms isolated from cow milk and are associated with subclinical mastitis and persistent increases in the bulk milk somatic cell count (BMSCC) of low BMSCC herds. By combining peptide enrichment, LC-ESI-MS/MS, and statistical analysis, we investigated the influence of subclinical mastitis and CNS infection on the milk peptidome. Quarter milk samples from clinically healthy Holstein cows were subjected to bacteriological culture (BC) and somatic cell counting (SCC) for two consecutive samplings and 28 (including 11 negatives and 17 positives) were selected for peptidomic analysis. The study identified 1363 different endogenous peptides and highlighted a significant increase of peptides in CNS-positive milk, mainly represented by casein fragments. Milk peptidome changes increased with the SCC, as also demonstrated by protein electrophoresis and densitometry. Peptides significantly different in CNS or CONTROL samples were identified and characterized. Our results indicate that subclinical mastitis by CNS can induce significant changes in the milk peptidome, opening the way to future studies for the identification of a biomarker panel as well as for the understanding of their consequences for the technological and sensorial characteristics of cow milk and dairy products.

Influence of subclinical mastitis and intramammary infection by coagulase-negative staphylococci on the cow milk peptidome / M.F. Addis, E.M. Maffioli, F. Ceciliani, G. Tedeschi, V. Zamarian, F.M. Tangorra, M. Albertini, R. Piccinini, V. Bronzo. - In: JOURNAL OF PROTEOMICS. - ISSN 1876-7737. - (2020 Jul 07). [Epub ahead of print]

Influence of subclinical mastitis and intramammary infection by coagulase-negative staphylococci on the cow milk peptidome

Maria Filippa Addis;Elisa Margherita Maffioli;Fabrizio Ceciliani;Gabriella Tedeschi;Valentina Zamarian;Francesco Tangorra;Mariangela Albertini;Renata Piccinini;Valerio Bronzo
2020-07-07

Abstract

Coagulase-negative staphylococci (CNS) are the most prevalent microorganisms isolated from cow milk and are associated with subclinical mastitis and persistent increases in the bulk milk somatic cell count (BMSCC) of low BMSCC herds. By combining peptide enrichment, LC-ESI-MS/MS, and statistical analysis, we investigated the influence of subclinical mastitis and CNS infection on the milk peptidome. Quarter milk samples from clinically healthy Holstein cows were subjected to bacteriological culture (BC) and somatic cell counting (SCC) for two consecutive samplings and 28 (including 11 negatives and 17 positives) were selected for peptidomic analysis. The study identified 1363 different endogenous peptides and highlighted a significant increase of peptides in CNS-positive milk, mainly represented by casein fragments. Milk peptidome changes increased with the SCC, as also demonstrated by protein electrophoresis and densitometry. Peptides significantly different in CNS or CONTROL samples were identified and characterized. Our results indicate that subclinical mastitis by CNS can induce significant changes in the milk peptidome, opening the way to future studies for the identification of a biomarker panel as well as for the understanding of their consequences for the technological and sensorial characteristics of cow milk and dairy products.
Subclinical mastitis; coagulase-negative staphylococci; milk; peptidomics; tandem mass spectrometry
Settore VET/05 - Malattie Infettive degli Animali Domestici
Settore BIO/10 - Biochimica
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
7-lug-2020
JOURNAL OF PROTEOMICS
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/749027
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