The MADS-domain transcription factor SEEDSTICK (STK) controls several aspects of plant reproduction. STK is co-expressed with CESTA (CES), a basic Helix-Loop-Helix (bHLH) transcription factor-encoding gene. CES was reported to control redundantly with the brassinosteroid positive signaling factors BRASSINOSTEROID ENHANCED EXPRESSION1 (BEE1) and BEE3 the development of the transmitting tract. Combining the stk ces-4 mutants led to a reduction in ovule fertilization due to a defect in carpel fusion which, caused the formation of holes at the center of the septum where the transmitting tract differentiates. Combining the stk mutant with the bee1 bee3 ces-4 triple mutant showed an increased number of unfertilized ovules and septum defects. The transcriptome profile of this quadruple mutant revealed a small subset of differentially expressed genes which are mainly involved in cell death, extracellular matrix and cell wall development. Our data evidence a regulatory gene network controlling transmitting tract development regulated directly or indirectly by a STK-CES containing complex and reveal new insights in the regulation of transmitting tract development by bHLH and MADS-domain transcription factors.

MADS-Box and bHLH Transcription Factors Coordinate Transmitting Tract Development in Arabidopsis thaliana / M. Di Marzo, I. Roig-Villanova, E. Zanchetti, F. Caselli, V. Gregis, P. Bardetti, M. Chiara, A. Guazzotti, E. Caporali, M.A. Mendes, L. Colombo, M.M. Kater. - In: FRONTIERS IN PLANT SCIENCE. - ISSN 1664-462X. - 11(2020 May 06).

MADS-Box and bHLH Transcription Factors Coordinate Transmitting Tract Development in Arabidopsis thaliana

Di Marzo, Maurizio;Roig-Villanova, Irma;Zanchetti, Eva;Caselli, Francesca;Gregis, Veronica;Chiara, Matteo;Guazzotti, Andrea;Caporali, Elisabetta;Mendes, Marta Adelina;Colombo, Lucia;Kater, Martin M.
2020-05-06

Abstract

The MADS-domain transcription factor SEEDSTICK (STK) controls several aspects of plant reproduction. STK is co-expressed with CESTA (CES), a basic Helix-Loop-Helix (bHLH) transcription factor-encoding gene. CES was reported to control redundantly with the brassinosteroid positive signaling factors BRASSINOSTEROID ENHANCED EXPRESSION1 (BEE1) and BEE3 the development of the transmitting tract. Combining the stk ces-4 mutants led to a reduction in ovule fertilization due to a defect in carpel fusion which, caused the formation of holes at the center of the septum where the transmitting tract differentiates. Combining the stk mutant with the bee1 bee3 ces-4 triple mutant showed an increased number of unfertilized ovules and septum defects. The transcriptome profile of this quadruple mutant revealed a small subset of differentially expressed genes which are mainly involved in cell death, extracellular matrix and cell wall development. Our data evidence a regulatory gene network controlling transmitting tract development regulated directly or indirectly by a STK-CES containing complex and reveal new insights in the regulation of transmitting tract development by bHLH and MADS-domain transcription factors.
bHLH, cell death, cell wall, extracellular matrix, MADS-box, transmitting tract
Settore BIO/01 - Botanica Generale
Settore BIO/18 - Genetica
Settore BIO/11 - Biologia Molecolare
6-mag- 202
FRONTIERS IN PLANT SCIENCE
https://www.frontiersin.org/articles/10.3389/fpls.2020.00526/full#h1
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/733265
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