The bi-enzymatic synthesis of the antiviral drug vidarabine (arabinosyladenine, ara-A), catalyzed by uridine phosphorylase from Clostridium perfringens (CpUP) and a purine nucleoside phosphorylase fromAeromonas hydrophila (AhPNP), was re-designed under continuous-flow conditions. Glyoxyl-agarose and EziGTM1 (Opal) were used as immobilization carriers for carrying out this preparative biotransformation. Upon setting-up reaction parameters (substrate concentration and molar ratio, temperature, pressure, residence time), 1 g of vidarabine was obtained in 55% isolated yield and >99% purity by simply running the flow reactor for 1 week and then collecting (by filtration) the nucleoside precipitated out of the exiting flow. Taking into account the substrate specificity of CpUP and AhPNP, the results obtained pave the way to the use of the CpUP/AhPNP-based bioreactor for the preparation of other purine nucleosides.

An enzymatic flow-based preparative route to vidarabine / L. Tamborini, C. Previtali, F. Annunziata, T. Bavaro, M. Terreni, E. Calleri, F. Rinaldi, A. Pinto, G. Speranza, D. Ubiali, P. Conti. - In: MOLECULES. - ISSN 1420-3049. - 25:5(2020 Mar), pp. 1223.1-1223.13. [10.3390/molecules25051223]

An enzymatic flow-based preparative route to vidarabine

L. Tamborini
Primo
;
C. Previtali;F. Annunziata;A. Pinto;G. Speranza;P. Conti
Ultimo
2020

Abstract

The bi-enzymatic synthesis of the antiviral drug vidarabine (arabinosyladenine, ara-A), catalyzed by uridine phosphorylase from Clostridium perfringens (CpUP) and a purine nucleoside phosphorylase fromAeromonas hydrophila (AhPNP), was re-designed under continuous-flow conditions. Glyoxyl-agarose and EziGTM1 (Opal) were used as immobilization carriers for carrying out this preparative biotransformation. Upon setting-up reaction parameters (substrate concentration and molar ratio, temperature, pressure, residence time), 1 g of vidarabine was obtained in 55% isolated yield and >99% purity by simply running the flow reactor for 1 week and then collecting (by filtration) the nucleoside precipitated out of the exiting flow. Taking into account the substrate specificity of CpUP and AhPNP, the results obtained pave the way to the use of the CpUP/AhPNP-based bioreactor for the preparation of other purine nucleosides.
Enzyme immobilization; Flow bioreactor; Nucleoside phosphorylase; Nucleosides; Vidarabine
Settore CHIM/08 - Chimica Farmaceutica
   BIOFLOW: an innovative platform for the in-flow biocatalytic preparation of high value chemicals
   FONDAZIONE CARIPLO
   2016-0731
mar-2020
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/731624
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