Aim: To study Weissella cibaria and Weissella confusa strains, lactic acid bacteria (LAB) members naturally present in food products, but not yet included in Qualified Presumption of Safety (QPS) list of European Food Safety Authority (EFSA). Methods and Results: We carried out a comparative genome analysis of 23 sequenced W. cibaria and 7 W. confusa genomes, in parallel with a physiological and functional characterization of several strains previously isolated from sourdough-like maize bran fermentation. The genome analysis revealed the absence of dedicated pathogenicity factors. Some putative virulence genes found in Weissella genomes were also present in other LAB strains, considered safe by EFSA and commonly used as probiotics. The physiological tests carried out on our strains corroborated the genomic results. Moreover, the following functional traits of interest to application in the food sector were identified: the majority of tested strains displayed high acidification rate, high reducing ability, production of exopolysaccharides (EPS), arabinoxylan degradation ability, growth in the presence of fructo-oligosaccharides (FOS), bile and gastric juice tolerance, and antifungal activity. Conclusions: These results provide evidence for the possible use of selected strains of W. cibaria and W. confusa in the food sector. Significance and Impact of the Study: This polyphasic study adds to the body of knowledge on the functional and applicable characteristics of these controversial species of LAB. This knowledge contributes to design new selected cultures included in the QPS list required for food applications.
A polyphasic approach to characterize Weissella cibaria and Weissella confusa strains / M. Quattrini, D. Korcari, G. Ricci, M.G. Fortina. - In: JOURNAL OF APPLIED MICROBIOLOGY. - ISSN 1364-5072. - 128:2(2020 Feb), pp. 500-512.
|Titolo:||A polyphasic approach to characterize Weissella cibaria and Weissella confusa strains|
QUATTRINI, MATTIA (Primo)
KORCARI, DEA (Secondo)
FORTINA, MARIA GRAZIA (Corresponding)
|Parole Chiave:||antifungal activity; comparative genomic analysis; functional characteristics; virulence traits; Weissella cibaria; Weissella confusa|
|Settore Scientifico Disciplinare:||Settore AGR/16 - Microbiologia Agraria|
|Data di pubblicazione:||feb-2020|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1111/jam.14483|
|Appare nelle tipologie:||01 - Articolo su periodico|
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