The cell surface properties of human intestinal bifidobacteria have been characterized for 30 strains isolated from a fecal sample. Strain identification to the species level was obtained by restriction analysis of the amplified 16S rRNA gene and confirmed by DNA/DNA reassociation experiments. The isolates were grouped in four genetically homogeneous clusters whose members belonged to Bifidobacterium bifidum, Bifidobacterium adolescentis, Bifidobacterium longum and Bifidobacterium pseudocatenulatum species. Cell surface properties of Bifidobacterium strains were evaluated by determining the level of hydrophobicity, adhesion to hydrocarbons and contact angle measurements, and their autoaggregation ability. The results showed high and homogeneous level of hydrophobicity in all tested strains when contact angle measurements values were considered. On the contrary, autoaggregation assays and bacterial adhesion to hydrocarbons detected interesting differences in cell surface properties among the tested Bifidobacterium strains. The highest levels of autoaggregation, detected in B. bifidum and B. adolescentis strains, were strictly dependent on the pH of the medium. Moreover, protease treatment experiments suggested that proteins had a key role in the autoaggregating ability of B. bifidum and B. adolescentis strains. (copyright) Springer 2005.

Conditions affecting cell surface properties of human intestinal bifidobacteria / E. Canzi, S.D. Guglielmetti, D. Mora, I. Tamagnini, C. Parini. - In: ANTONIE VAN LEEUWENHOEK. - ISSN 0003-6072. - 88:3-4(2005 Oct), pp. 207-219.

Conditions affecting cell surface properties of human intestinal bifidobacteria

E. Canzi
Primo
;
S.D. Guglielmetti
Secondo
;
D. Mora;C. Parini
Ultimo
2005-10

Abstract

The cell surface properties of human intestinal bifidobacteria have been characterized for 30 strains isolated from a fecal sample. Strain identification to the species level was obtained by restriction analysis of the amplified 16S rRNA gene and confirmed by DNA/DNA reassociation experiments. The isolates were grouped in four genetically homogeneous clusters whose members belonged to Bifidobacterium bifidum, Bifidobacterium adolescentis, Bifidobacterium longum and Bifidobacterium pseudocatenulatum species. Cell surface properties of Bifidobacterium strains were evaluated by determining the level of hydrophobicity, adhesion to hydrocarbons and contact angle measurements, and their autoaggregation ability. The results showed high and homogeneous level of hydrophobicity in all tested strains when contact angle measurements values were considered. On the contrary, autoaggregation assays and bacterial adhesion to hydrocarbons detected interesting differences in cell surface properties among the tested Bifidobacterium strains. The highest levels of autoaggregation, detected in B. bifidum and B. adolescentis strains, were strictly dependent on the pH of the medium. Moreover, protease treatment experiments suggested that proteins had a key role in the autoaggregating ability of B. bifidum and B. adolescentis strains. (copyright) Springer 2005.
Bifidobacteriaceae ; Bifidobacterium adolescentis ; Bifidobacterium bifidum ; Bifidobacterium longum ; Bifidobacterium ; DNA recombination ; adult ; article ; bacterial gene ; bacterial strain ; bacterium adherence ; bifidobacterium pseudocatenulatum ; cell surface ; contact angle ; feces analysis ; female ; gene amplification ; gene cluster ; human ; hydrophobicity ; intestine flora ; nonhuman ; priority journal ; protein function ; restriction mapping ; strain difference ; strain identification ; RNA 16S ; bacterial DNA ; bacterial RNA ; bacterial protein ; hydrocarbon ; proteinase
Settore AGR/16 - Microbiologia Agraria
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/6721
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