Polycomb repressive complexes 1 and 2 (PRC1 and PRC2) control cell identity by establishing facultative heterochromatin repressive domains at common sets of target genes. PRC1, which deposits H2Aub1 through the E3 ligases RING1A/B, forms six biochemically distinct subcomplexes depending on the assembled PCGF protein (PCGF1-PCGF6); however, it is yet unclear whether these subcomplexes have also specific activities. Here we show that PCGF1 and PCGF2 largely compensate for each other, while other PCGF proteins have high levels of specificity for distinct target genes. PCGF2 associates with transcription repression, whereas PCGF3 and PCGF6 associate with actively transcribed genes. Notably, PCGF3 and PCGF6 complexes can assemble and be recruited to several active sites independently of RING1A/B activity (therefore, of PRC1). For chromatin recruitment, the PCGF6 complex requires the combinatorial activities of its MGA-MAX and E2F6-DP1 subunits, while PCGF3 requires an interaction with the USF1 DNA binding transcription factor.

Functional Landscape of PCGF Proteins Reveals Both RING1A/B-Dependent-and RING1A/B-Independent-Specific Activities / A. Scelfo, D. Fernandez-Perez, S. Tamburri, M. Zanotti, E. Lavarone, M. Soldi, T. Bonaldi, K.J. Ferrari, D. Pasini. - In: MOLECULAR CELL. - ISSN 1097-2765. - 74:5(2019 Jun 06), pp. 1037-1052. [10.1016/j.molcel.2019.04.002]

Functional Landscape of PCGF Proteins Reveals Both RING1A/B-Dependent-and RING1A/B-Independent-Specific Activities

A. Scelfo
Co-primo
;
D. Fernandez-Perez
Co-primo
;
S. Tamburri
Co-primo
;
M. Zanotti
Secondo
;
T. Bonaldi;K.J. Ferrari
Penultimo
;
D. Pasini
Ultimo
2019

Abstract

Polycomb repressive complexes 1 and 2 (PRC1 and PRC2) control cell identity by establishing facultative heterochromatin repressive domains at common sets of target genes. PRC1, which deposits H2Aub1 through the E3 ligases RING1A/B, forms six biochemically distinct subcomplexes depending on the assembled PCGF protein (PCGF1-PCGF6); however, it is yet unclear whether these subcomplexes have also specific activities. Here we show that PCGF1 and PCGF2 largely compensate for each other, while other PCGF proteins have high levels of specificity for distinct target genes. PCGF2 associates with transcription repression, whereas PCGF3 and PCGF6 associate with actively transcribed genes. Notably, PCGF3 and PCGF6 complexes can assemble and be recruited to several active sites independently of RING1A/B activity (therefore, of PRC1). For chromatin recruitment, the PCGF6 complex requires the combinatorial activities of its MGA-MAX and E2F6-DP1 subunits, while PCGF3 requires an interaction with the USF1 DNA binding transcription factor.
Polycomb group proteins; repressive complex 2; histone H3; gene-expression; genomic targets; H2A; transcription; recruitment; methylation; binding
Settore BIO/11 - Biologia Molecolare
   Dissecting the Function of Multiple Polycomb Group Complexes in Establishing Transcriptional Identity (Dissect PcG)
   Dissect PcG
   EUROPEAN COMMISSION
   H2020
   755268
6-giu-2019
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/661519
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