Polycomb repressive complexes 1 and 2 (PRC1 and PRC2) control cell identity by establishing facultative heterochromatin repressive domains at common sets of target genes. PRC1, which deposits H2Aub1 through the E3 ligases RING1A/B, forms six biochemically distinct subcomplexes depending on the assembled PCGF protein (PCGF1-PCGF6); however, it is yet unclear whether these subcomplexes have also specific activities. Here we show that PCGF1 and PCGF2 largely compensate for each other, while other PCGF proteins have high levels of specificity for distinct target genes. PCGF2 associates with transcription repression, whereas PCGF3 and PCGF6 associate with actively transcribed genes. Notably, PCGF3 and PCGF6 complexes can assemble and be recruited to several active sites independently of RING1A/B activity (therefore, of PRC1). For chromatin recruitment, the PCGF6 complex requires the combinatorial activities of its MGA-MAX and E2F6-DP1 subunits, while PCGF3 requires an interaction with the USF1 DNA binding transcription factor.
Functional Landscape of PCGF Proteins Reveals Both RING1A/B-Dependent-and RING1A/B-Independent-Specific Activities / A. Scelfo, D. Fernandez-Perez, S. Tamburri, M. Zanotti, E. Lavarone, M. Soldi, T. Bonaldi, K.J. Ferrari, D. Pasini. - In: MOLECULAR CELL. - ISSN 1097-2765. - 74:5(2019 Jun 06), pp. 1037-1052.
|Titolo:||Functional Landscape of PCGF Proteins Reveals Both RING1A/B-Dependent-and RING1A/B-Independent-Specific Activities|
SCELFO, ANDREA (Co-primo)
FERNANDEZ PEREZ, DANIEL (Co-primo)
TAMBURRI, SIMONE (Co-primo)
ZANOTTI, MARTA (Secondo)
FERRARI, KARIN JOHANNA (Penultimo) (Corresponding)
PASINI, DIEGO (Ultimo) (Corresponding)
|Parole Chiave:||Polycomb group proteins; repressive complex 2; histone H3; gene-expression; genomic targets; H2A; transcription; recruitment; methylation; binding|
|Settore Scientifico Disciplinare:||Settore BIO/11 - Biologia Molecolare|
|Progetto:||Dissecting the Function of Multiple Polycomb Group Complexes in Establishing Transcriptional Identity (Dissect PcG)|
|Data di pubblicazione:||6-giu-2019|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.molcel.2019.04.002|
|Appare nelle tipologie:||01 - Articolo su periodico|