A Polymerase Chain Reaction (PCR) method was set up to determine the ratio between pig (Sus scrofa) and goose (Anser anser) DNA quantities in a mixture. A "universal" primer and two specific primers for goose and pig were used. A highly significant statistical correlation (P ∼ 0.01) between goose and pig DNA quantities and the electrophoretic band areas of PCR products was found.
Use of Polymerase Chain Reaction (PCR) and electrophoretic gel computer-assisted statistical analysis to semi-quantitatively determine pig/goose DNA ratio / F. Colombo, E. Marchisio, C. Cantoni. - In: ITALIAN JOURNAL OF FOOD SCIENCE. - ISSN 1120-1770. - 14:1(2002), pp. 71-76.
Use of Polymerase Chain Reaction (PCR) and electrophoretic gel computer-assisted statistical analysis to semi-quantitatively determine pig/goose DNA ratio
F. ColomboPrimo
;C. CantoniUltimo
2002
Abstract
A Polymerase Chain Reaction (PCR) method was set up to determine the ratio between pig (Sus scrofa) and goose (Anser anser) DNA quantities in a mixture. A "universal" primer and two specific primers for goose and pig were used. A highly significant statistical correlation (P ∼ 0.01) between goose and pig DNA quantities and the electrophoretic band areas of PCR products was found.
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