IRIS Institutional Research Information System - AIR Archivio Istituzionale della Ricerca
IRIS è il sistema di gestione integrata dei dati della ricerca (persone, progetti, pubblicazioni, attività) adottato dall'Università degli Studi di Milano.
AIR nasce in UNIMI nel 2006 con lo scopo di raccogliere, documentare e conservare le informazioni sulla produzione scientifica dell'Università degli Studi di Milano e costituisce di fatto l'Anagrafe della ricerca dell'Ateneo.
EnglishHuman endothelial cells (ECs) have been employed to monitor the protein changes induced by [3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one] (3PO), a compound able to inhibit the glycolytic flux partially and transiently and to reduce pathological angiogenesis in a variety of disease models. Normal and TNFα induced inflamed ECs were incubated with and without 3PO at a concentration (20 μM) able to inhibit cell proliferation without cell death. At the end of the incubation period, samples were submitted to the following steps: (a) whole protein extraction, reduction, alkylation, and digestion by trypsin; (b) peptide separation by nano-LC-MS/MS analysis using a high-resolution mass spectrometer; (c) data analysis including protein identification, quantification, and statistical analysis. An altered protein expression profiling in combination with protein network analysis was employed by using a mass spectrometry-based label-free quantification approach to explore the underlying mechanisms of 3PO at cellular level.
Mass Spectrometry-based Label-free Quantitative Proteomics to Study the Effect of 3PO Drug at Cellular Level / S.B. Nukala, G. Baron, G. Aldini, M. Carini, A. D'Amato. - In: ACS MEDICINAL CHEMISTRY LETTERS. - ISSN 1948-5875. - 10:4 (special issue)(2019 Apr), pp. 577-583.
| Titolo: | Mass Spectrometry-based Label-free Quantitative Proteomics to Study the Effect of 3PO Drug at Cellular Level |
| Autori: | BARON, GIOVANNA (Secondo) CARINI, MARINA (Penultimo) D'AMATO, ALFONSINA (Ultimo) (Corresponding) |
| Parole Chiave: | 3PO; cell pathway modulation; inflammation; mass spectrometry; PFKFB3 inhibitor; Quantitative proteomics; Biochemistry; Drug Discovery3003 Pharmaceutical Science; Organic Chemistry |
| Settore Scientifico Disciplinare: | Settore CHIM/01 - Chimica Analitica Settore BIO/10 - Biochimica Settore CHIM/08 - Chimica Farmaceutica |
| Progetto: | Modulation of glycolytic flux as a new approach for treatment of atherosclerosis and plaque stabilization: a multidisciplinary study (MOGLYNET) |
| Data di pubblicazione: | apr-2019 |
| Rivista: | |
| Tipologia: | Article (author) |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1021/acsmedchemlett.8b00593 |
| Appare nelle tipologie: | 01 - Articolo su periodico |
File in questo prodotto:
| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| acsmedchemlett.8b00593.pdf | Publisher's version/PDF | Administrator Richiedi una copia | ||
| Nukalaetal_ACS_postprint.pdf | 3PO_ACS_post_print | Post-print, accepted manuscript ecc. (versione accettata dall'editore) | Open Access Visualizza/Apri |
Copyright © 2021