Reactive carbonyl species (RCS) are cytotoxic molecules that originate from lipid peroxidation and sugar oxidation. Natural derivatives can be an attractive source of potential RCS scavenger. However, the lack of analytical methods to screen and identify bioactive compounds contained in complex matrices has hindered their identification. The sequestering actions of various rice extracts on RCS have been determined using ubiquitin and 4-hydroxy-2-nonenal (HNE) as a protein and RCS model, respectively. Black rice with giant embryo extract was found to be the most effective among various rice varieties. The identification of bioactive compounds was then carried out by an isotopic signature profile method using the characteristic isotopic ion cluster generated by the mixture of HNE:2H5-HNE mixed at a 1:1 stoichiometric ratio. An in-house database was used to obtain the structures of the possible bioactive components. The identified compounds were further confirmed as HNE sequestering agents through HPLC-UV analysis.
Isotopic labelling for the characterisation of HNE-sequestering agents in plant-based extracts and its application for the identification of anthocyanidins in black rice with giant embryo / M. Colzani, L. Regazzoni, A. Criscuolo, G. Baron, M. Carini, G. Vistoli, Y. Lee, S. Han, G. Aldini, K. Yeum. - In: FREE RADICAL RESEARCH. - ISSN 1071-5762. - 52:8(2018 Aug), pp. 896-906.
|Titolo:||Isotopic labelling for the characterisation of HNE-sequestering agents in plant-based extracts and its application for the identification of anthocyanidins in black rice with giant embryo|
COLZANI, MARA (Primo)
REGAZZONI, LUCA GIOVANNI (Secondo)
ALDINI, GIANCARLO (Penultimo) (Corresponding)
|Parole Chiave:||Black rice with giant embryo; HNE sequestering agents; mass spectrometry; Biochemistry|
|Settore Scientifico Disciplinare:||Settore CHIM/08 - Chimica Farmaceutica|
|Data di pubblicazione:||ago-2018|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1080/10715762.2018.1490735|
|Appare nelle tipologie:||01 - Articolo su periodico|
File in questo prodotto:
|Isotopic labelling for the characterisation of HNE sequestering agents in plant based extracts and its application for the identification of.pdf||Publisher's version/PDF||Administrator Richiedi una copia|
|Black_rice_final_version_postprint.pdf||Post-print, accepted manuscript ecc. (versione accettata dall'editore)||Open Access Visualizza/Apri|