Lactococcus garvieae is now recognized as a species with clinical significance for human and veterinary medicine. The aim of this study was to evaluate the presence of this pathogen in sand bedding and milk samples. Two farms in Minnesota with problems of clinical and subclinical mastitis due to streptococci-like organisms were selected. Twenty-four Lactococcus garvieae isolates from sand bedding and 18 isolates from quarter milk were comparatively studied using a genotypic approach. RAPD (random amplification of polymorphic DNA) PCR and REP (repetitive element palindromic) PCR experiments highlighted a similar electrophoretic profile. When genes belonging to the core genome of L. garvieae were tested through a MLRT (multilocus restriction typing), we again observed that all L. garvieae isolates coming from sand bedding and milk shared a common profile, distinguishable from previously studied representative L. garvieae strains. These data indicate that the L. garvieae isolated from sand bedding and milk originated from a few strains adapted to persist in the same habitat. This supports the hypothesis that sand bedding can represent a reservoir of L. garvieae strains and be a potential vehicle for their dissemination in dairy farms.

Sand bedding as a reservoir for Lactococcus garvieae dissemination in dairy farms / G. Eraclio, G. Ricci, P. Moroni, C. Santisteban, C. Plumed-Ferrer, J. Bennett, M.G. Fortina. - In: CANADIAN JOURNAL OF MICROBIOLOGY. - ISSN 0008-4166. - 65:1(2019 Jan), pp. 84-89. [10.1139/cjm-2018-0251]

Sand bedding as a reservoir for Lactococcus garvieae dissemination in dairy farms

G. Eraclio
Primo
;
G. Ricci;P. Moroni;M.G. Fortina
Ultimo
2019

Abstract

Lactococcus garvieae is now recognized as a species with clinical significance for human and veterinary medicine. The aim of this study was to evaluate the presence of this pathogen in sand bedding and milk samples. Two farms in Minnesota with problems of clinical and subclinical mastitis due to streptococci-like organisms were selected. Twenty-four Lactococcus garvieae isolates from sand bedding and 18 isolates from quarter milk were comparatively studied using a genotypic approach. RAPD (random amplification of polymorphic DNA) PCR and REP (repetitive element palindromic) PCR experiments highlighted a similar electrophoretic profile. When genes belonging to the core genome of L. garvieae were tested through a MLRT (multilocus restriction typing), we again observed that all L. garvieae isolates coming from sand bedding and milk shared a common profile, distinguishable from previously studied representative L. garvieae strains. These data indicate that the L. garvieae isolated from sand bedding and milk originated from a few strains adapted to persist in the same habitat. This supports the hypothesis that sand bedding can represent a reservoir of L. garvieae strains and be a potential vehicle for their dissemination in dairy farms.
Lactococcus garvieae est maintenant considéré comme une espèce d’importance clinique chez l’humain et en médecine vétérinaire. L’objectif de cette étude consistait à évaluer la présence de cet agent pathogène dans de la litière de sable et des échantillons de lait. Deux fermes du Minnesota qui présentaient des problèmes de mammite clinique et subclinique provoquée par des organismes de type streptococcus ont été sélectionnées. Vingt-quatre isolats de Lactococcus garvieae de la litière de sable et 18 isolats de lait du pis ont été étudiés de manière comparative à l’aide d’une approche génotypique. Des expériences en RAPD (« random amplification of polymorphic DNA ») et en REP (« repetitive element palindromic ») PCR mettaient en évidence des profils électrophorétiques similaires. Lorsque les gènes appartenant au génome central de L. garvieae étaient testés par MLRT (« multilocus restriction typing »), les auteurs observaient encore que tous les isolats de L. garvieae provenant de la litière de sable et du lait partageaient un profil commun, distinctif de celui de souches de L. garvieae représentatives, étudiées précédemment. Ces données indiquent que L. garvieae isolé de la litière de sable et du lait proviennent d’un nombre limité de souches adaptées pour persister dans le même habitat. Ceci appuie l’hypothèse que la litière de sable peut constituer un réservoir de souches de L. garvieae et un véhicule de leur dissémination dans les fermes laitières.
MLRT; dissémination environnementale; environmental dissemination; lait; milk; litière de sable; sand bedding; molecular typing; typage moléculaire
Settore AGR/16 - Microbiologia Agraria
Settore VET/05 - Malattie Infettive degli Animali Domestici
gen-2019
18-set-2018
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/595885
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