An improved procedure for the microbial hydroxylations of dehydroepiandrosterone (DHEA, 1) and 15β,16β-methylene-dehydroepiandrosterone (2) was studied using whole cells of Botryodiplodia malorum and Colletotrichum lini. C. lini catalyzed 7α- and 15α-hydroxylation of 1 and 7α-hydroxylation of 2, while B. malorum gave 7β-hydroxylation of both the substrates. The stability of the enzymatic activity was higher in the presence of co-substrates (i.e., glucose or mannitol) allowing for repeated batches of the biotransformations. The yields of 7α,15α-dihydroxy-1 production were improved obtaining 5.8 g l−1 (recovered product) from 7.0 g l−1 of substrate. The structures of the hydroxylated products were assigned by a combination of two-dimensional NMR proton–proton and proton–carbon correlation techniques.
|Titolo:||Steroid hydroxylations with Botryodiplodia malorum and Colletotrichum lini|
|Autori interni:||RAGG, ENZIO MARIA|
MOLINARI, FRANCESCO ENZO (Ultimo)
GANDOLFI, RAFFAELLA (Penultimo)
ROMANO, DIEGO (Secondo)
ROMANO, ANDREA (Primo)
|Parole Chiave:||steroids, biotransformation,Botryodiplodia malorum, Colletotrichum lini|
|Settore Scientifico Disciplinare:||Settore CHIM/11 - Chimica e Biotecnologia delle Fermentazioni|
Settore CHIM/06 - Chimica Organica
|Data di pubblicazione:||2006|
|Digital Object Identifier (DOI):||10.1016/j.steroids.2006.01.014|
|Appare nelle tipologie:||01 - Articolo su periodico|
File in questo prodotto:
- PubMed Central loading...