In this study, a comparison between elderly (>70 years) and young subjects reveals that elder people are subject to a higher oxidative stress, which causes an increase in plasma hydroperoxide levels (18%) and a decrease in antioxidant defenses (25%). Moreover, the marked decrease of the erythrocyte membrane fluidity observed in elderly subjects was likely to affect the behavior of some membrane glycohydrolases. In fact, a significant decrease of beta-d-glucuronidase and neutral sialidase (30 and 50%, respectively) was detected. Activity differences were also observed when erythrocytes were further distinguished according to their biological age. Striking differences between young and elderly subjects were observed for beta-d-glucuronidase and neutral sialidase in young and senescent erythrocytes, respectively. Overall beta-d-glucuronidase decreases with the subjects' age, while neutral sialidase levels are higher in the elderly. This is presumably due to the localization of these enzymes in distinct plasma membrane micro-domains, which are differently peroxidized. A possible role of these enzymes in signaling praecox membrane alterations has also been evidenced.
Erythrocyte membrane alterations during ageing affect beta-D-glucuronidase and neutral sialidase in elderly healthy subjects / G. Goi, R. Cazzola, C.A. Tringali, L. Massaccesi, S. Russo Volpe, M. Rondanelli, E. Ferrari, C.J. Baquero Herrera, B.A. Cestaro, A. Lombardo, B. Venerando. - In: EXPERIMENTAL GERONTOLOGY. - ISSN 0531-5565. - 40:3(2005), pp. 219-225.
|Titolo:||Erythrocyte membrane alterations during ageing affect beta-D-glucuronidase and neutral sialidase in elderly healthy subjects|
GOI, GIANCARLO (Primo)
CAZZOLA, ROBERTA (Secondo)
LOMBARDO, ADRIANA (Penultimo)
VENERANDO, BRUNO (Ultimo)
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
Settore ING-IND/11 - Fisica Tecnica Ambientale
Settore MED/46 - Scienze Tecniche di Medicina di Laboratorio
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
|Data di pubblicazione:||2005|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.exger.2004.08.013|
|Appare nelle tipologie:||01 - Articolo su periodico|