A robust two-enzyme system composed of an immobilized ketoreductase (KRED1-Pglu) and a glucose dehydrogenase (BmGDH) was developed via immobilization on aldehyde agarose for the stereoselective reduction of different ketones. The immobilized ketoreductase/glucose dehydrogenase system was continuously used in a flow reactor for weeks, even in the presence of concentrations of DMSO up to 20%.
Flow-based stereoselective reduction of ketones using an immobilized ketoreductase/glucose dehydrogenase mixed bed system / F. Dall'Oglio, M.L. Contente, P. Conti, F. Molinari, D. Monfredi, A. Pinto, D. Romano, D. Ubiali, L. Tamborini, I. Serra. - In: CATALYSIS COMMUNICATIONS. - ISSN 1566-7367. - 93:(2017), pp. 29-32. [10.1016/j.catcom.2017.01.025]
Flow-based stereoselective reduction of ketones using an immobilized ketoreductase/glucose dehydrogenase mixed bed system
F. Dall'Oglio;M.L. ContenteSecondo
;P. Conti;F. Molinari;A. Pinto;D. Romano;L. TamboriniPenultimo
;I. SerraUltimo
2017
Abstract
A robust two-enzyme system composed of an immobilized ketoreductase (KRED1-Pglu) and a glucose dehydrogenase (BmGDH) was developed via immobilization on aldehyde agarose for the stereoselective reduction of different ketones. The immobilized ketoreductase/glucose dehydrogenase system was continuously used in a flow reactor for weeks, even in the presence of concentrations of DMSO up to 20%.
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Catalysis Communications 2017 Dall'Oglio.pdf
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