We describe aerogels obtained by laccase/TEMPO-oxidation and lyophilization of galactomannans (GM) from fenugreek, sesbania and guar. Enzymatic oxidation of GM in aqueous solution caused viscosity increase up to fifteen-fold, generating structured, elastic, stable hydrogels, presumably due to establishment of hemiacetalic bonds between newly formed carbonyl groups and free hydroxyl groups. Upon lyophilization, water-insoluble aerogels were obtained, whose mechanical properties and porosity were investigated. Active principles were absorbed into the aerogels from aqueous solutions and, following rinsing, blotting, re-lyophilization, were released in an appropriate medium. The release of the antibiotic polymyxin B was tested against six different Gram-negative bacterial strains, of the antimicrobial peptide nisin against two Gram-positive and of the muraminidase lysozyme against one anaerobic strain. Protease and lipase release in solution from “enzyme loaded” aerogels was monitored by the increase in enzymatic activity. These biomaterials could represent new versatile, biocompatible “delivery systems” of actives for biomedical and industrial applications.

Aerogels of enzymatically oxidized galactomannans from leguminous plants: Versatile delivery systems of antimicrobial peptides and enzymes / P. Campia, E. Ponzini, B. Rossi, S. Farris, T. Silvetti, L. Merlini, M. Brasca, R. Grandori, Y.M. Galante. - In: CARBOHYDRATE POLYMERS. - ISSN 0144-8617. - 158(2017), pp. 102-111. [10.1016/j.carbpol.2016.11.089]

Aerogels of enzymatically oxidized galactomannans from leguminous plants: Versatile delivery systems of antimicrobial peptides and enzymes

S. Farris;T. Silvetti;
2017

Abstract

We describe aerogels obtained by laccase/TEMPO-oxidation and lyophilization of galactomannans (GM) from fenugreek, sesbania and guar. Enzymatic oxidation of GM in aqueous solution caused viscosity increase up to fifteen-fold, generating structured, elastic, stable hydrogels, presumably due to establishment of hemiacetalic bonds between newly formed carbonyl groups and free hydroxyl groups. Upon lyophilization, water-insoluble aerogels were obtained, whose mechanical properties and porosity were investigated. Active principles were absorbed into the aerogels from aqueous solutions and, following rinsing, blotting, re-lyophilization, were released in an appropriate medium. The release of the antibiotic polymyxin B was tested against six different Gram-negative bacterial strains, of the antimicrobial peptide nisin against two Gram-positive and of the muraminidase lysozyme against one anaerobic strain. Protease and lipase release in solution from “enzyme loaded” aerogels was monitored by the increase in enzymatic activity. These biomaterials could represent new versatile, biocompatible “delivery systems” of actives for biomedical and industrial applications.
Galactomannans; Polymyxin; Nisin; Lysozyme; Aerogels; Delivery systems
Settore AGR/15 - Scienze e Tecnologie Alimentari
2-dic-2016
Article (author)
File in questo prodotto:
File Dimensione Formato  
Pre-print.pdf

accesso aperto

Tipologia: Pre-print (manoscritto inviato all'editore)
Dimensione 4.41 MB
Formato Adobe PDF
4.41 MB Adobe PDF Visualizza/Apri
1-s2.0-S0144861716313674-main.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 1.37 MB
Formato Adobe PDF
1.37 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/467361
Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 20
  • ???jsp.display-item.citation.isi??? 15
social impact