Dimeric/oligomeric states of G-protein coupled receptors have been difficult to target. We report here bivalent ligands consisting of two identical oxytocin-mimetics that induce a three order magnitude boost in G-protein signaling of oxytocin receptors (OTRs) in vitro and a 100- and 40-fold gain in potency in vivo in the social behavior of mice and zebrafish. Through receptor mutagenesis and interference experiments with synthetic peptides mimicking transmembrane helices (TMH), we show that such superpotent behavior follows from the binding of the bivalent ligands to dimeric receptors based on a TMH1-TMH2 interface. Moreover, in this arrangement, only the analogues with a well-defined spacer length (∼25 Å) precisely fit inside a channel-like passage between the two protomers of the dimer. The newly discovered oxytocin bivalent ligands represent a powerful tool for targeting dimeric OTR in neurodevelopmental and psychiatric disorders and, in general, provide a framework to untangle specific arrangements of G-protein coupled receptor dimers.

Design and characterization of superpotent bivalent ligands targeting oxytocin receptor dimers via a channel-like structure / M. Busnelli, G. Kleinau, M. Muttenthaler, S. Stoev, M. Manning, L. Bibic, L.A. Howell, P.J. Mccormick, S. Di Lascio, D. Braida, M. Sala, G.E. Rovati, T.G. Bellini, B. Chini. - In: JOURNAL OF MEDICINAL CHEMISTRY. - ISSN 0022-2623. - 59:15(2016 Aug 11), pp. 7152-7166. [10.1021/acs.jmedchem.6b00564]

Design and characterization of superpotent bivalent ligands targeting oxytocin receptor dimers via a channel-like structure

M. Busnelli
Primo
;
S. DI LASCIO;D. Braida;M. Sala;G.E. Rovati;T.G. Bellini
Penultimo
;
2016

Abstract

Dimeric/oligomeric states of G-protein coupled receptors have been difficult to target. We report here bivalent ligands consisting of two identical oxytocin-mimetics that induce a three order magnitude boost in G-protein signaling of oxytocin receptors (OTRs) in vitro and a 100- and 40-fold gain in potency in vivo in the social behavior of mice and zebrafish. Through receptor mutagenesis and interference experiments with synthetic peptides mimicking transmembrane helices (TMH), we show that such superpotent behavior follows from the binding of the bivalent ligands to dimeric receptors based on a TMH1-TMH2 interface. Moreover, in this arrangement, only the analogues with a well-defined spacer length (∼25 Å) precisely fit inside a channel-like passage between the two protomers of the dimer. The newly discovered oxytocin bivalent ligands represent a powerful tool for targeting dimeric OTR in neurodevelopmental and psychiatric disorders and, in general, provide a framework to untangle specific arrangements of G-protein coupled receptor dimers.
protein-coupled receptors; single-molecule analysis; crystal-structure; allosteric interactions; beta(2)-adrenergic receptor; ERK1/2 activation; social-behavior; in-vitro; agonists; GPCR
Settore BIO/14 - Farmacologia
11-ago-2016
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/430357
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