The accumulation dynamics of cellulolytic enzymes in culture media of the basidiomycete fungi Panus tigrinus, Pleurotus ostreatus, Fomes fomentarus, and the micromycete Aspergillus terreus were studied during a long incubation period. It was found that A. terreus was the most active producer of cellulolytic enzymes among the studied fungi. Two protein fractions with cellulase activity were isolated using gel filtration and ion-exchange chromatography. PAAG electrophoresis showed that fraction-I consisted of four components; fraction-II, an electrophoretically homogeneous protein.
Isolation, purification, and enzymatic activity of cellulase components of the fungus Aspergillus terreus / S. Y. Mirzaakhmedov, Z. F. Ziyavitdinov, Z. R. Akhmedova, A. B. Saliev, D. T. Ruzmetova, K. B. Ashurov, D. Fessas, S. Iametti. - In: CHEMISTRY OF NATURAL COMPOUNDS. - ISSN 0009-3130. - 43:5(2007), pp. 594-597.
Isolation, purification, and enzymatic activity of cellulase components of the fungus Aspergillus terreus
D. FessasPenultimo
;S. IamettiUltimo
2007
Abstract
The accumulation dynamics of cellulolytic enzymes in culture media of the basidiomycete fungi Panus tigrinus, Pleurotus ostreatus, Fomes fomentarus, and the micromycete Aspergillus terreus were studied during a long incubation period. It was found that A. terreus was the most active producer of cellulolytic enzymes among the studied fungi. Two protein fractions with cellulase activity were isolated using gel filtration and ion-exchange chromatography. PAAG electrophoresis showed that fraction-I consisted of four components; fraction-II, an electrophoretically homogeneous protein.
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