Shoots of the apple cultivar Golden Delicious clone B were maintained actively proliferating for several years and routinely used as source of leaves to regenerate adventitious shoots. Contamination appearing during proliferation did not affect the performances of axillary branching or shoot growth but was further related to a dramatic decrease of regenerative ability of the leaves. Two bacterial strains, C1 and C2, were isolated and identified on the basis of the 16S rRNA gene partial sequence analysis. C1 strain showed significant homology with Luteibacter, a gamma-proteobacterium isolated from the rhizosphere of Hordeum vulgare, while C2 showed the highest similarity with Staphylococcus pasteuri. Leaves artificially infected with either single C1 and C2 cells or both strains together recorded a significant drop in shoot regeneration ability and concealed the leaf morphogenic gradient. On the other hand, contaminated leaves produced more abundant callus that could result from the production of growth regulators by the bacterial strains.

Identification And Effect of Two Bacterial Contaminants on Apple Organogenesis / M.C. Piagnani, S. Guglielmetti, C. Parini. - In: ACTA HORTICULTURAE. - ISSN 0567-7572. - 738(2007), pp. 335-340. ((Intervento presentato al convegno ISHS International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species tenutosi a Daytona Beach, Florida nel 2005.

Identification And Effect of Two Bacterial Contaminants on Apple Organogenesis

M.C. Piagnani
Primo
;
S. Guglielmetti
Secondo
;
C. Parini
Ultimo
2007

Abstract

Shoots of the apple cultivar Golden Delicious clone B were maintained actively proliferating for several years and routinely used as source of leaves to regenerate adventitious shoots. Contamination appearing during proliferation did not affect the performances of axillary branching or shoot growth but was further related to a dramatic decrease of regenerative ability of the leaves. Two bacterial strains, C1 and C2, were isolated and identified on the basis of the 16S rRNA gene partial sequence analysis. C1 strain showed significant homology with Luteibacter, a gamma-proteobacterium isolated from the rhizosphere of Hordeum vulgare, while C2 showed the highest similarity with Staphylococcus pasteuri. Leaves artificially infected with either single C1 and C2 cells or both strains together recorded a significant drop in shoot regeneration ability and concealed the leaf morphogenic gradient. On the other hand, contaminated leaves produced more abundant callus that could result from the production of growth regulators by the bacterial strains.
'Golden delicious; Callus; Caulogenesis; Luteibacter; Regeneration; Staphylococcus
Settore AGR/16 - Microbiologia Agraria
Settore AGR/03 - Arboricoltura Generale e Coltivazioni Arboree
USDA ARS, Kearneysville, WV
USDA ARS Subtropical Horticultural Research Center, Miami, FL
University of Florida/IFAS
Article (author)
File in questo prodotto:
File Dimensione Formato  
paper bacteria.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 126.99 kB
Formato Adobe PDF
126.99 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/40465
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 2
  • ???jsp.display-item.citation.isi??? 2
social impact