To study the hepatic expression of genes involved in lipid metabolism in periparturient dairy goats fed diets enriched with saturated or unsaturated fatty acids was the main aim of the trial. Twenty three second parity alpine dairy goats were assigned to three treatments: (C; n=8) fed a non fat-supplemented basal diet, (ST; n=7) fed a basal diet supplemented with stearic acid and (FO; n=8) fed a basal diet supplemented with fish oil. The supplementation started from the last week of gestation and lasted 21 days after kidding and supplied 30g/head/d extra fatty acids during the dry period and 50g/head/d during lactation. Liver biopsied samples were harvested at day -7, 7 and 21 relative to kidding date and immediately snap-frozen. Blood samples were collected on days -7, -2, 0, 2, 7, 14 and 21. Quantitative real-time RT-PCR of ACAA1, ACOX1, SCD, SOD, CAT, SREBF2 and PPARA was performed. Data obtained were analyzed using a MIXED procedure of SAS. No differences were observed on milk pr oduction, milk composition, body weight and body condition score. SCD expression was significantly (1.8-fold; P<0.01) up-regulated in ST at days 7 and 21 while the opposite trend was observed in FO. ACOX1 pattern was similar for both fat-supplemented diets peaking at day 7 (ST 1.6-fold; P<0.05) and returning close to neutrality at 21 day. NEFA serum content was high in FO and ST at day 2 and remained higher in FO at days 7 and 14 (P<0.05). Cholesterol peaked in C at day 7 and in FO at days 14 and 21 (P<0.05). SCD expression pattern could explain higher levels of free cholesterol, since has been proposed its role in cholesterol esterification to MUFA. Results support a modulation of hepatic lipid metabolism by saturated and unsaturated dietary fat supplements.
Liver gene expression in periparturient dairy goats fed diets enriched with stearate or PUFA / J.M. Caputo, G. Invernizzi, M. Ferroni, A. Agazzi, J.J. Loor, V. Dell'Orto, G. Savoini - In: Book of Abstracts of the 65th Annual Meeting of the European Federation of Animal ScienceWageningen : Wageningen Academic Publishers, 2014. - ISBN 9789086862481. - pp. 402-402 (( Intervento presentato al 65. convegno Annual Meeting of the European Federation of Animal Science tenutosi a Copenhaghen nel 2014.
|Titolo:||Liver gene expression in periparturient dairy goats fed diets enriched with stearate or PUFA|
CAPUTO, JESSICA MICHELA (Primo)
INVERNIZZI, GUIDO (Secondo)
DELL'ORTO, VITTORIO (Penultimo)
SAVOINI, GIOVANNI (Ultimo)
|Settore Scientifico Disciplinare:||Settore AGR/18 - Nutrizione e Alimentazione Animale|
|Data di pubblicazione:||2014|
|Enti collegati al convegno:||European Federation of Animal Science (EAAP)|
|Tipologia:||Book Part (author)|
|Appare nelle tipologie:||03 - Contributo in volume|