This work describes a sensitive high-performance liquid chromatography (HPLC) method for the quantification of aloesin and aloeresin A in alcoholic beverages containing aloe as a flavoring agent. The compounds were prepared from Aloe ferox juice. Sephadex LH20 and ion-exchange resin AG1X2 column chromatography were used for aloesin. Aloeresin A was obtained by Sephadex LH20 and silica gel column chromatography followed by purification on Discovery DSC-18 solid-phase extraction tubes. A 98 mg amount of aloesin (>99% purity) and 34 mg of aloeresin A (>98% purity) were recovered from 2.5 g of aloe juice. The HPLC method was validated, and intra- and interday performances were established. In-house validation was carried out by analyzing samples of beverages with and without aloe as a flavoring agent.
Determination of Aloesin and Aloeresin A for the detection of Aloe in Beverage / M. Dell'Agli, F. Giavarini, P. Ferraboschi, G. Galli, E.A. Bosisio. - In: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. - ISSN 0021-8561. - 55:9(2007), pp. 3363-3367.
|Titolo:||Determination of Aloesin and Aloeresin A for the detection of Aloe in Beverage|
DELL'AGLI, MARIO (Primo)
GIAVARINI, FLAVIO (Secondo)
GALLI, GIOVANNI CARLO (Penultimo)
BOSISIO, ENRICA ANGELA (Ultimo)
|Parole Chiave:||Aloesin ; aloeresin A ; C-glucosylchromones ; aloe juice ; hydroalcoholic beverage ; Aloe ferox|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
Settore BIO/15 - Biologia Farmaceutica
|Data di pubblicazione:||2007|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1021/jf070182h|
|Appare nelle tipologie:||01 - Articolo su periodico|