Air artificially contaminated with increasing concentrations of benzene was treated in a laboratory scale compost-packed biofilter for 240 days with a removal efficiency of 81-100%. The bacterial community in the packing material (PM) at different heights of the biofilter was analysed every 60 days. Bacterial plate counts and ribosomal intergenic spacer analysis (RISA) of the isolated strains showed that the number of cultivable aerobic heterotrophic bacteria and the species diversity increased with benzene availability. Identification of the isolated species and the main bands in denaturing gradient gel electrophoresis (DGGE) profiles from total compost DNA during the treatment revealed that, at a relatively low volumetric benzene load (1.2 <= VBL <= 6.4 g m(PM)(-3) h(-1)), besides low G+C Gram positive bacteria, originally present in the packing compost, bacteroidetes and beta- and gamma-proteobacteria became detectable in the colonising population. At the VBL value (24.8 g m(PM)(-3) h(-1)) ensuring the maximum elimination capacity of the biofilter (20.1 g m(PM)(-3) h(-1)), strains affiliated to the genus Rhodococcus dominated the micro. ora, followed by beta-proteobacteria comprising the genera Bordetella and Neisseria. Under these conditions, more than 35% of the isolated strains were able to grow on benzene as the sole carbon source. Comparison of DGGE and automated RISA profiles of the total community and isolated strains showed that a complex bacterial succession occurred in the reactor in response to the increasing concentrations of the pollutant and that cultivable bacteria played a major role in benzene degradation under the adopted conditions.

Microbial succession in a compost-packed biofilter treating benzene-contaminated air / S. Borin, M. Marzorati, L. Brusetti, M. Zilli, H. Cherif, A. Hassen, A. Converti, C. Sorlini, D. Daffonchio. - In: BIODEGRADATION. - ISSN 0923-9820. - 17:2(2006 Mar), pp. 79-89. [10.1007/s10532-005-7565-5]

Microbial succession in a compost-packed biofilter treating benzene-contaminated air

S. Borin
Primo
;
M. Marzorati
Secondo
;
L. Brusetti;C. Sorlini
Penultimo
;
D. Daffonchio
Ultimo
2006

Abstract

Air artificially contaminated with increasing concentrations of benzene was treated in a laboratory scale compost-packed biofilter for 240 days with a removal efficiency of 81-100%. The bacterial community in the packing material (PM) at different heights of the biofilter was analysed every 60 days. Bacterial plate counts and ribosomal intergenic spacer analysis (RISA) of the isolated strains showed that the number of cultivable aerobic heterotrophic bacteria and the species diversity increased with benzene availability. Identification of the isolated species and the main bands in denaturing gradient gel electrophoresis (DGGE) profiles from total compost DNA during the treatment revealed that, at a relatively low volumetric benzene load (1.2 <= VBL <= 6.4 g m(PM)(-3) h(-1)), besides low G+C Gram positive bacteria, originally present in the packing compost, bacteroidetes and beta- and gamma-proteobacteria became detectable in the colonising population. At the VBL value (24.8 g m(PM)(-3) h(-1)) ensuring the maximum elimination capacity of the biofilter (20.1 g m(PM)(-3) h(-1)), strains affiliated to the genus Rhodococcus dominated the micro. ora, followed by beta-proteobacteria comprising the genera Bordetella and Neisseria. Under these conditions, more than 35% of the isolated strains were able to grow on benzene as the sole carbon source. Comparison of DGGE and automated RISA profiles of the total community and isolated strains showed that a complex bacterial succession occurred in the reactor in response to the increasing concentrations of the pollutant and that cultivable bacteria played a major role in benzene degradation under the adopted conditions.
Air biofiltration ; benzene ; compost ; microbial ecology ; DNA fingerprinting ; Rhodococcus
Settore AGR/16 - Microbiologia Agraria
mar-2006
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/31223
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