Trigeminal (TG) pain often lacks a satisfactory pharmacological control. A better understanding of the molecular cross-talk between TG neurons and surrounding satellite glial cells (SGCs) could help identifying innovative targets for the development of more effective analgesics. We have previously demonstrated that neuronal pro-algogenic mediators upregulate G protein-coupled nucleotide P2Y receptors (P2YRs) expressed by TG SGCs in vitro. Here, we have identified the specific P2YR subtypes involved (i.e., the ADP-sensitive P2Y<inf>1</inf>R and the UTP-responsive P2Y<inf>2</inf>R subtypes), and demonstrated the contribution of neuron-derived prostaglandins to their upregulation. Next, we have translated these data to an in vivo model of TG pain (namely, rats injected with Complete Freund's adjuvant in the temporomandibular joint), by demonstrating activation of SGCs and upregulation of P2Y<inf>1</inf>R and P2Y<inf>2</inf>R in the ipsi-lateral TG. To unequivocally link P2YRs to the development of facial allodynia, we treated animals with various purinergic antagonists. The selective P2Y<inf>2</inf>R antagonist AR-C118925 completely inhibited SGCs activation, exerted a potent anti-allodynic effect that lasted over time, and was still effective when administration was started 6-days post induction of allodynia, i.e. under subchronic pain conditions. Conversely, the selective P2Y<inf>1</inf>R antagonist MRS2179 was completely ineffective. Moreover, similarly to the anti-inflammatory drug acetylsalicylic acid and the known anti-migraine agent sumatriptan, the P2X/P2Y nonselective antagonist PPADS was only partially effective, and completely lost its activity under sub-chronic conditions. Taken together, our results highlight glial P2Y<inf>2</inf>Rs as potential "druggable" targets for the successful management of TG-related pain. GLIA 2015;63:1256-1269 Main Points: Pro-algogenic mediators modulate P2Y2 purinergic receptors on trigeminal satellite glial cells in vitro; Selective blockade of P2Y2 receptors reverts allodynia and satellite glial cell activation in an in vivo model of trigeminal sensitization.

P2Y2 receptor antagonists as anti-allodynic agents in acute and sub-chronic trigeminal sensitization: role of satellite glial cells / G. Magni, D. Merli, C. Verderio, M.P. Abbracchio, S. Ceruti. - In: GLIA. - ISSN 0894-1491. - 63:7(2015 Jul), pp. 1256-1269. [10.1002/glia.22819]

P2Y2 receptor antagonists as anti-allodynic agents in acute and sub-chronic trigeminal sensitization: role of satellite glial cells

G. Magni
Primo
;
D. Merli
Secondo
;
M.P. Abbracchio
Penultimo
;
S. Ceruti
Ultimo
2015

Abstract

Trigeminal (TG) pain often lacks a satisfactory pharmacological control. A better understanding of the molecular cross-talk between TG neurons and surrounding satellite glial cells (SGCs) could help identifying innovative targets for the development of more effective analgesics. We have previously demonstrated that neuronal pro-algogenic mediators upregulate G protein-coupled nucleotide P2Y receptors (P2YRs) expressed by TG SGCs in vitro. Here, we have identified the specific P2YR subtypes involved (i.e., the ADP-sensitive P2Y1R and the UTP-responsive P2Y2R subtypes), and demonstrated the contribution of neuron-derived prostaglandins to their upregulation. Next, we have translated these data to an in vivo model of TG pain (namely, rats injected with Complete Freund's adjuvant in the temporomandibular joint), by demonstrating activation of SGCs and upregulation of P2Y1R and P2Y2R in the ipsi-lateral TG. To unequivocally link P2YRs to the development of facial allodynia, we treated animals with various purinergic antagonists. The selective P2Y2R antagonist AR-C118925 completely inhibited SGCs activation, exerted a potent anti-allodynic effect that lasted over time, and was still effective when administration was started 6-days post induction of allodynia, i.e. under subchronic pain conditions. Conversely, the selective P2Y1R antagonist MRS2179 was completely ineffective. Moreover, similarly to the anti-inflammatory drug acetylsalicylic acid and the known anti-migraine agent sumatriptan, the P2X/P2Y nonselective antagonist PPADS was only partially effective, and completely lost its activity under sub-chronic conditions. Taken together, our results highlight glial P2Y2Rs as potential "druggable" targets for the successful management of TG-related pain. GLIA 2015;63:1256-1269 Main Points: Pro-algogenic mediators modulate P2Y2 purinergic receptors on trigeminal satellite glial cells in vitro; Selective blockade of P2Y2 receptors reverts allodynia and satellite glial cell activation in an in vivo model of trigeminal sensitization.
AR-C118925; Orofacial pain; P2Y<inf>2</inf> receptors; Satellite glial cells; Trigeminal pain
Settore BIO/14 - Farmacologia
lug-2015
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/276848
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