Introduction: Platelet mitochondrial respiratory chain enzymes (that produce energy) are variably inhibited during human sepsis. Whether these changes occur even during other acute critical illness or are associated with impaired platelet aggregation and secretion (that consume energy) is not known. The aims of this study were firstly to compare platelet mitochondrial respiratory chain enzymes activity between patients with sepsis and those with cardiogenic shock, and secondly to study the relationship between platelet mitochondrial respiratory chain enzymes activity and platelet responsiveness to (exogenous) agonists in patients with sepsis. Methods: This was a prospective, observational, case-control study. Platelets were isolated from venous blood of 16 patients with severe sepsis or septic shock (free from antiplatelet drugs) and 16 others with cardiogenic shock, within 48 hours from admission to Intensive Care. Platelet mitochondrial respiratory chain enzymes activity was measured with spectrophotometry and expressed relative to citrate synthase activity, a marker of mitochondrial density. Platelet aggregation and secretion in response to adenosine di-phosphate (ADP), collagen, U46619 and thrombin receptor activating peptide were measured with lumiaggregometry only in patients with sepsis. In total, 16 healthy volunteers acted as controls for both spectrophotometry and lumiaggregometry. Results: Platelets of patients with sepsis or cardiogenic shock similarly had lower mitochondrial nicotinamide adenine dinucleotide dehydrogenase (NADH) (P<0.001), complex I (P=0.006), complex I and III (P<0.001) and complex IV (P<0.001) activity than those of controls. Platelets of patients with sepsis were generally hypo-responsive to exogenous agonists, both in terms of maximal aggregation (P<0.001) and secretion (P<0.05). Lower mitochondrial NADH (R 2 0.36; P<0.001), complex I (R2 0.38; P<0.001), complex I and III (R2 0.27; P=0.002) and complex IV (R2 0.43; P<0.001) activity was associated with lower first wave of aggregation with ADP. Conclusions: Several platelet mitochondrial respiratory chain enzymes are similarly inhibited during human sepsis and cardiogenic shock. In patients with sepsis, mitochondrial dysfunction is associated with general platelet hypo-responsiveness to exogenous agonists.

Platelet mitochondrial dysfunction in critically ill patients: comparison between sepsis and cardiogenic shock / A. Protti, F. Fortunato, A. Artoni, A. Lecchi, G. Motta, G. Mistraletti, C. Novembrino, P. Comi, L. Gattinoni. - In: CRITICAL CARE. - ISSN 1364-8535. - 19:1(2015), pp. 39.-39.1. [10.1186/s13054-015-0762-7]

Platelet mitochondrial dysfunction in critically ill patients: comparison between sepsis and cardiogenic shock

A. Protti
;
F. Fortunato
Secondo
;
A. Artoni;G. Mistraletti;C. Novembrino;P. Comi
Penultimo
;
L. Gattinoni
Ultimo
2015

Abstract

Introduction: Platelet mitochondrial respiratory chain enzymes (that produce energy) are variably inhibited during human sepsis. Whether these changes occur even during other acute critical illness or are associated with impaired platelet aggregation and secretion (that consume energy) is not known. The aims of this study were firstly to compare platelet mitochondrial respiratory chain enzymes activity between patients with sepsis and those with cardiogenic shock, and secondly to study the relationship between platelet mitochondrial respiratory chain enzymes activity and platelet responsiveness to (exogenous) agonists in patients with sepsis. Methods: This was a prospective, observational, case-control study. Platelets were isolated from venous blood of 16 patients with severe sepsis or septic shock (free from antiplatelet drugs) and 16 others with cardiogenic shock, within 48 hours from admission to Intensive Care. Platelet mitochondrial respiratory chain enzymes activity was measured with spectrophotometry and expressed relative to citrate synthase activity, a marker of mitochondrial density. Platelet aggregation and secretion in response to adenosine di-phosphate (ADP), collagen, U46619 and thrombin receptor activating peptide were measured with lumiaggregometry only in patients with sepsis. In total, 16 healthy volunteers acted as controls for both spectrophotometry and lumiaggregometry. Results: Platelets of patients with sepsis or cardiogenic shock similarly had lower mitochondrial nicotinamide adenine dinucleotide dehydrogenase (NADH) (P<0.001), complex I (P=0.006), complex I and III (P<0.001) and complex IV (P<0.001) activity than those of controls. Platelets of patients with sepsis were generally hypo-responsive to exogenous agonists, both in terms of maximal aggregation (P<0.001) and secretion (P<0.05). Lower mitochondrial NADH (R 2 0.36; P<0.001), complex I (R2 0.38; P<0.001), complex I and III (R2 0.27; P=0.002) and complex IV (R2 0.43; P<0.001) activity was associated with lower first wave of aggregation with ADP. Conclusions: Several platelet mitochondrial respiratory chain enzymes are similarly inhibited during human sepsis and cardiogenic shock. In patients with sepsis, mitochondrial dysfunction is associated with general platelet hypo-responsiveness to exogenous agonists.
adult; aged; Article; blood sampling; cardiogenic shock; case control study; cell density; cellular secretion; clinical article; controlled study; critically ill patient; disease severity; disorders of mitochondrial functions; enzyme activity; female; human; intensive care; male; observational study; priority journal; prospective study; respiratory chain; sepsis; septic shock; spectrophotometry; thrombocyte; thrombocyte aggregation
Settore MED/41 - Anestesiologia
Settore MED/09 - Medicina Interna
Settore MED/26 - Neurologia
Article (author)
File in questo prodotto:
File Dimensione Formato  
s13054-015-0762-7.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 470.92 kB
Formato Adobe PDF
470.92 kB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/267665
Citazioni
  • ???jsp.display-item.citation.pmc??? 18
  • Scopus 34
  • ???jsp.display-item.citation.isi??? 33
social impact