An intracellular bacterium with the unique ability to enter mitochondria exists in the European vector of Lyme disease, the hard tick Ixodes ricinus. Previous phylogenetic analyses based on 16S rRNA gene sequences suggested that the bacterium formed a divergent lineage within the Rickettsiales (Alphaproteobacteria). Here, we present additional phylogenetic evidence, based on the gyrB gene sequence, that confirms the phylogenetic position of the bacterium, Based on these data, as well as electron microscopy (EM), in situ hybridization and other observations, we propose the name 'Candidatus Miclichloria mitochondrii' for this bacterium. The symbiont appears to be ubiquitous in females of L ricinus across the tick's distribution, while lower prevalence is observed in males (44%). Based on EM and in situ hybridization studies, the presence of 'Candidatus M. mitochondrii' in females appears to be restricted to ovarian cells. The bacterium was found to be localized both in the cytoplasm and in the intermembrane space of the mitochondria of ovarian cells. 'Candidatus M. mitochondrii' is the first bacterium to be identified that resides within animal mitochondria.

'Candidatus Midichloria mitochondrii', an endosymbiont of the tick Ixodes ricinus with a unique intramitochondrial lifestyle / D. Sassera, T. Beninati, C. Bandi, E. A. P. Bouman, L. Sacchi, M. Fabbi, N. Lo,. - In: INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. - ISSN 1466-5026. - 56:11(2006), pp. 2535-2540. [10.1099/ijs.0.64386-0]

'Candidatus Midichloria mitochondrii', an endosymbiont of the tick Ixodes ricinus with a unique intramitochondrial lifestyle

D. Sassera
Primo
;
T. Beninati;C. Bandi;N. Lo
Ultimo
2006

Abstract

An intracellular bacterium with the unique ability to enter mitochondria exists in the European vector of Lyme disease, the hard tick Ixodes ricinus. Previous phylogenetic analyses based on 16S rRNA gene sequences suggested that the bacterium formed a divergent lineage within the Rickettsiales (Alphaproteobacteria). Here, we present additional phylogenetic evidence, based on the gyrB gene sequence, that confirms the phylogenetic position of the bacterium, Based on these data, as well as electron microscopy (EM), in situ hybridization and other observations, we propose the name 'Candidatus Miclichloria mitochondrii' for this bacterium. The symbiont appears to be ubiquitous in females of L ricinus across the tick's distribution, while lower prevalence is observed in males (44%). Based on EM and in situ hybridization studies, the presence of 'Candidatus M. mitochondrii' in females appears to be restricted to ovarian cells. The bacterium was found to be localized both in the cytoplasm and in the intermembrane space of the mitochondria of ovarian cells. 'Candidatus M. mitochondrii' is the first bacterium to be identified that resides within animal mitochondria.
Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali
2006
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/26012
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