Objective: We propose a fluorescence kinetics method for monitoring plasma susceptibility to peroxidation. Design and Method: Plasmatic peroxidation was induced by CuSO4 (500 mu M), and fluorescence was measured every 30 min. Kinetics were represented by a sigmoidal curve from which it was possible to calculate the latency time (lag-time) and the propagation velocity (slope) of plasma peroxidation. Results: The lag-time monitored by the fluorescence kinetics method corresponded to the formation of thiobarbituric acid reactive substances, and to progressive depletion of polyunsaturated fatty acids and alpha-tocopherol. The mechanism of reaction appeared to be dependent upon plasmatic hydroperoxides, and independent of oxygen radicals. Plasma storage is possible for at least two months at -80 degrees C, and reproducibility of the method is very good. Conclusions: Fluorescence kinetics provide a highly comprehensive picture of plasma susceptibility to peroxidation in comparison with the conventional measurements of anti- and prooxidant ratios

A fluorescence method for the determination of plasma susceptibility to lipid peroxidation / G. Cervato, P. Viani, R. Cazzola, B. Cestaro. - In: CLINICAL BIOCHEMISTRY. - ISSN 0009-9120. - 32:3(1999 Apr), pp. 171-177.

A fluorescence method for the determination of plasma susceptibility to lipid peroxidation

G. Cervato
Primo
;
P. Viani
Secondo
;
R. Cazzola
Penultimo
;
B. Cestaro
Ultimo
1999

Abstract

Objective: We propose a fluorescence kinetics method for monitoring plasma susceptibility to peroxidation. Design and Method: Plasmatic peroxidation was induced by CuSO4 (500 mu M), and fluorescence was measured every 30 min. Kinetics were represented by a sigmoidal curve from which it was possible to calculate the latency time (lag-time) and the propagation velocity (slope) of plasma peroxidation. Results: The lag-time monitored by the fluorescence kinetics method corresponded to the formation of thiobarbituric acid reactive substances, and to progressive depletion of polyunsaturated fatty acids and alpha-tocopherol. The mechanism of reaction appeared to be dependent upon plasmatic hydroperoxides, and independent of oxygen radicals. Plasma storage is possible for at least two months at -80 degrees C, and reproducibility of the method is very good. Conclusions: Fluorescence kinetics provide a highly comprehensive picture of plasma susceptibility to peroxidation in comparison with the conventional measurements of anti- and prooxidant ratios
Vitamin E; Plasma; Fatty Acids, Unsaturated; Spectrometry, Fluorescence; Reproducibility of Results; Kinetics; Humans; Thiobarbituric Acid Reactive Substances; Free Radical Scavengers; Copper; Lipid Peroxidation
Settore BIO/10 - Biochimica
apr-1999
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/258734
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