In this work, a novel injectable biocomposite hydrogel is produced by internal gelation, using pectin as organic matrix and hydroxyapatite either as crosslinking agent and inorganic reinforcement. Tunable gelling kinetics and rheological properties are obtained varying the hydrogels' composition, with the final aim of developing systems for cell immobilization. The reversibility by dissolution of pectin-hydroxyapatite hydrogels is achieved with saline solutions, to possibly accelerate the release of the cells or active agents immobilized. Texture analysis confirms the possibility of extruding the biocomposites from needles with diameters from 20 G to 30 G, indicating that they can be implanted with minimally-invasive approaches, minimizing the pain during injection and the side effects of the open surgery. L929 fibroblasts entrapped in the hydrogels survive to the immobilization procedure and exhibit high cell viability. On the overall, these systems result to be suitable supports for the immobilization of cells for tissue regeneration applications.

Reactive hydroxyapatite fillers for pectin biocomposites / F. Munarin, P. Petrini, G. Barcellona, T. Roversi, L. Piazza, L. Visai, M.C. Tanzi. - In: MATERIALS SCIENCE AND ENGINEERING. C, BIOMIMETIC MATERIALS, SENSORS AND SYSTEMS. - ISSN 0928-4931. - 45(2014), pp. 154-161. [10.1016/j.msec.2014.09.003]

Reactive hydroxyapatite fillers for pectin biocomposites

T. Roversi;L. Piazza;
2014

Abstract

In this work, a novel injectable biocomposite hydrogel is produced by internal gelation, using pectin as organic matrix and hydroxyapatite either as crosslinking agent and inorganic reinforcement. Tunable gelling kinetics and rheological properties are obtained varying the hydrogels' composition, with the final aim of developing systems for cell immobilization. The reversibility by dissolution of pectin-hydroxyapatite hydrogels is achieved with saline solutions, to possibly accelerate the release of the cells or active agents immobilized. Texture analysis confirms the possibility of extruding the biocomposites from needles with diameters from 20 G to 30 G, indicating that they can be implanted with minimally-invasive approaches, minimizing the pain during injection and the side effects of the open surgery. L929 fibroblasts entrapped in the hydrogels survive to the immobilization procedure and exhibit high cell viability. On the overall, these systems result to be suitable supports for the immobilization of cells for tissue regeneration applications.
Biomaterials; Hydrogels; Hydroxyapatite; Injectable; Internal gelation; Pectin; Materials Science (all); Condensed Matter Physics; Mechanical Engineering; Mechanics of Materials
Settore ING-IND/22 - Scienza e Tecnologia dei Materiali
Settore AGR/15 - Scienze e Tecnologie Alimentari
2014
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/254580
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