The paper provides a simple protocol that uses the polymerase chain reaction to amplify a specific portion of the 16S gene, allowing the recognition of Pseudomonas fluorescens from other group I Pseudomonas. The amplified DNA patterns of 16S rRNA and ITS1, from the restriction fragment length polymorphisms VspI, HaeIII and TaqI digestion, produced band patterns that distinguished the biotypes of Ps. fluorescens. In addition to distinguishing the biotypes C and 3 we used a phenotypical method for levan production.
Development of PCR assay to identify Pseudomonas fluorescens and its biotype / M. Scarpellini, L. Franzetti, A. Galli. - In: FEMS MICROBIOLOGY LETTERS. - ISSN 0378-1097. - 236:2(2004 Jun 09), pp. 257-260. [10.1111/j.1574-6968.2004.tb09655.x]
Development of PCR assay to identify Pseudomonas fluorescens and its biotype
M. ScarpelliniPrimo
;L. FranzettiSecondo
;
2004
Abstract
The paper provides a simple protocol that uses the polymerase chain reaction to amplify a specific portion of the 16S gene, allowing the recognition of Pseudomonas fluorescens from other group I Pseudomonas. The amplified DNA patterns of 16S rRNA and ITS1, from the restriction fragment length polymorphisms VspI, HaeIII and TaqI digestion, produced band patterns that distinguished the biotypes of Ps. fluorescens. In addition to distinguishing the biotypes C and 3 we used a phenotypical method for levan production.Pubblicazioni consigliate
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