Sixty-four strains of Enterococcus spp. of different origin were identified using traditional phenotypic, biochemical and cultural tests, together with molecular tests. API 20 STREP tests identified the species at a preliminary level, only one strain remaining unidentified. Strains belonging to the genus Enterococcus were tested with genus-specific primers, while species-level identification was carried out with the 16S-23S rDNA intergenic region (ITS) and species-specific primers for E. faecium and E. faecalis. Those strains found to be negative with the species-specific primers were subjected to 16S rDNA partial sequencing.
Phenotypic and Genotypic Characterization of Enterococcus spp. of Different Origins / L. Franzetti, M. Pompei, M. Scarpellini, A. Galli. - In: CURRENT MICROBIOLOGY. - ISSN 0343-8651. - 49:4(2004 Mar 12), pp. 255-260. [10.1007/s00284-004-4242-6]
Phenotypic and Genotypic Characterization of Enterococcus spp. of Different Origins
L. FranzettiPrimo
;M. ScarpelliniPenultimo
;
2004
Abstract
Sixty-four strains of Enterococcus spp. of different origin were identified using traditional phenotypic, biochemical and cultural tests, together with molecular tests. API 20 STREP tests identified the species at a preliminary level, only one strain remaining unidentified. Strains belonging to the genus Enterococcus were tested with genus-specific primers, while species-level identification was carried out with the 16S-23S rDNA intergenic region (ITS) and species-specific primers for E. faecium and E. faecalis. Those strains found to be negative with the species-specific primers were subjected to 16S rDNA partial sequencing.Pubblicazioni consigliate
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