Sixty-four strains of Enterococcus spp. of different origin were identified using traditional phenotypic, biochemical and cultural tests, together with molecular tests. API 20 STREP tests identified the species at a preliminary level, only one strain remaining unidentified. Strains belonging to the genus Enterococcus were tested with genus-specific primers, while species-level identification was carried out with the 16S-23S rDNA intergenic region (ITS) and species-specific primers for E. faecium and E. faecalis. Those strains found to be negative with the species-specific primers were subjected to 16S rDNA partial sequencing.
|Titolo:||Phenotypic and Genotypic Characterization of Enterococcus spp. of Different Origins|
FRANZETTI, LAURA (Primo)
SCARPELLINI, MAURO (Penultimo)
|Settore Scientifico Disciplinare:||Settore AGR/16 - Microbiologia Agraria|
|Data di pubblicazione:||12-mar-2004|
|Digital Object Identifier (DOI):||10.1007/s00284-004-4242-6|
|Appare nelle tipologie:||01 - Articolo su periodico|