A screening among bacterial strains isolated from water-brine interface of the deep hypersaline anoxic basins (DHABs) of the Eastern Mediterranean was carried out for the biocatalytical resolution of racemic propyl ester of anti-2-oxotricyclo[2.2.1.0]heptan-7-carboxylic acid (R,S)-1, a key intermediate for the synthesis of d-cloprostenol. Bacillus horneckiae 15A gave highly stereoselective reduction of (R,S)-1, whereas Halomonas aquamarina 9B enantioselectively hydrolysed (R,S)-1; in both cases, enantiomerically pure unreacted (R)-1 could be easily recovered and purified at molar conversion below 57–58 %, showing the potential of DHAB extremophile microbiome and marine-derived enzymes in stereoselective biocatalysis.
Marine microorganisms as source of stereoselective esterases and ketoreductases : kinetic resolution of a prostaglandin intermediate / V. De Vitis, B. Guidi, M.L. Contente, T. Granato, P. Conti, F. Molinari, E. Crotti, F. Mapelli, S. Borin, D. Daffonchio, D. Romano. - In: MARINE BIOTECHNOLOGY. - ISSN 1436-2228. - 17:2(2015), pp. 144-152.
Titolo: | Marine microorganisms as source of stereoselective esterases and ketoreductases : kinetic resolution of a prostaglandin intermediate |
Autori: | GUIDI, BENEDETTA (Secondo) DAFFONCHIO, DANIELE GIUSEPPE (Penultimo) ROMANO, DIEGO (Ultimo) |
Parole Chiave: | Biocatalysis; Deep-sea hypersaline anoxic basins (DHABs); Esterase; Ketoreductase; Marine enzymes; Prostaglandin; Stereoselective |
Settore Scientifico Disciplinare: | Settore CHIM/11 - Chimica e Biotecnologia delle Fermentazioni Settore CHIM/08 - Chimica Farmaceutica Settore AGR/16 - Microbiologia Agraria |
Data di pubblicazione: | 2015 |
Rivista: | |
Tipologia: | Article (author) |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1007/s10126-014-9602-z |
Appare nelle tipologie: | 01 - Articolo su periodico |
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