The non-conventional yeast Pichia glucozyma CBS 5766 has been used for the biotransformation of different aromatic ketones and ketoesters. The growth and biotransformation conditions were optimised for the reduction of acetophenone and under optimised conditions, propiophenone, butyrophenone and valerophenone were reduced to the corresponding (S)-alcohols with high yields and enantioselectivity. Ketoreductase(s) of Pichia glucozyma showed high catalytic activities also towards aromatic β- and γ-ketoesters, being often competitive with esterase(s). These concurrent activities allowed for the preparation of hydroxyesters, hydroxyacids and lactones often in a very selective manner.
Biotransformation of aromatic ketones and ketoesters with the non-conventional yeast Pichia glucozyma / M.L. Contente, F. Molinari, P. Zambelli, V. De Vitis, R. Gandolfi, A. Pinto, D. Romano. - In: TETRAHEDRON LETTERS. - ISSN 0040-4039. - 55:51(2014), pp. 7051-7053.
Titolo: | Biotransformation of aromatic ketones and ketoesters with the non-conventional yeast Pichia glucozyma |
Autori: | CONTENTE, MARTINA LETIZIA (Primo) MOLINARI, FRANCESCO ENZO (Secondo) PINTO, ANDREA (Penultimo) ROMANO, DIEGO (Corresponding) |
Parole Chiave: | Aromatic ketones; Aromatic ketoesters; Reduction; Enantioselective; Ketoreductase; Esterase |
Settore Scientifico Disciplinare: | Settore CHIM/08 - Chimica Farmaceutica Settore CHIM/11 - Chimica e Biotecnologia delle Fermentazioni |
Data di pubblicazione: | 2014 |
Rivista: | |
Tipologia: | Article (author) |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/j.tetlet.2014.10.133 |
Appare nelle tipologie: | 01 - Articolo su periodico |
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