MPN-culture and MPN-PCR methods were used to determine Escherichia coli concentration in 54 retail minced meat samples. The MPN-PCR is based on the combination between the conventional Most Probable Number and the Polymerase Chain Reaction technique targeting the uidA gene. The conventional MPN-culture technique revealed about 94% of the samples to be positive for E. coli, while MPN-PCR showed only 87%; this difference in detection can be attributed to different factors. MPN-PCR showed values of relative accuracy, sensitivity and specificity of, respectively, 85,8%, 81,6% and 93,5%, and the analysis took only one day, compared with the 4-6 days needed using MPN-culture. The alternative method is reliable when microrganisms are present at low concentration levels.

Exploiting a MPN-PCR technique to quantify Escherichia coli in minced meat / R. Foschino, C. Picozzi, P. Di Pilato. - In: ANNALS OF MICROBIOLOGY. - ISSN 1590-4261. - 54:2(2004 Jun 30), pp. 99-105.

Exploiting a MPN-PCR technique to quantify Escherichia coli in minced meat

R. Foschino;C. Picozzi;
2004-06-30

Abstract

MPN-culture and MPN-PCR methods were used to determine Escherichia coli concentration in 54 retail minced meat samples. The MPN-PCR is based on the combination between the conventional Most Probable Number and the Polymerase Chain Reaction technique targeting the uidA gene. The conventional MPN-culture technique revealed about 94% of the samples to be positive for E. coli, while MPN-PCR showed only 87%; this difference in detection can be attributed to different factors. MPN-PCR showed values of relative accuracy, sensitivity and specificity of, respectively, 85,8%, 81,6% and 93,5%, and the analysis took only one day, compared with the 4-6 days needed using MPN-culture. The alternative method is reliable when microrganisms are present at low concentration levels.
Escherichia coli; MPN-PCR; minced meat; uidA
Settore AGR/16 - Microbiologia Agraria
ANNALS OF MICROBIOLOGY
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/23883
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