Purified lipase fractions from crude commercial extract of Aspergillus niger lipase (ANL) were selectively immobilized on hydrophobic supports. A significant percentage of esterase activity remains in the supernatant, derived from esterase(s) unable to become adsorbed onto the employed matrices. These residual proteins were covalently immobilized on epoxy-acrylic resin. Immobilized hydrolases were tested in the hydrolysis of acetylated disaccharides in water-cosolvent systems. ANL-Esterase was able to catalyze regioselective deprotection of acetylated b-O-methyl lactoside in C-2 position and b-O-methyl lactosaminide in C-3’ position. The hydrolyzed products, never reported before, can be considered new building blocks for the synthesis of oligosaccharides of biological relevance. Furthermore, preparative hydrolyses were also performed in tert-butanol. This solvent is compatible with ANL-esterase stability and it appears to be a novel and promising approach because of its green status.
|Titolo:||Regioselective Deacetylation of Disaccharides via Immobilized Aspergillus niger Esterase(s)-catalyzed Hydrolysis in Aqueous and Non-aqueous Media|
SPERANZA, GIOVANNA (Penultimo)
|Parole Chiave:||biocatalysis ; hydrolysis ; oligosaccharides ; regioselectivity ; supported catalysts|
|Settore Scientifico Disciplinare:||Settore CHIM/06 - Chimica Organica|
|Data di pubblicazione:||2013|
|Digital Object Identifier (DOI):||10.1002/cctc.201300388|
|Appare nelle tipologie:||01 - Articolo su periodico|