Cell-bound lipases of dry mycelium of Aspergillus oryzae were used in organic solvent for the resolution of racemic flurbiprofen by direct esterification with ethanol in a flow-chemistry reactor. Under flow conditions a significant reduction of the reaction time and an increase of the enantioselectivity were achieved compared to the batch mode. Moreover, the process was implemented by adding an in-line purification step integrated with the racemization of the unreacted flurbiprofen directly into a polymer-supported resin.

Biotransformation with whole microbial systems in a continuous flow reactor : resolution of (RS)-flurbiprofen using Aspergillus oryzae by direct esterification with ethanol in organic solvent / L. Tamborini, D. Romano, A. Pinto, M. Contente, M.C. Iannuzzi, P. Conti, F. Molinari. - In: TETRAHEDRON LETTERS. - ISSN 0040-4039. - 54:45(2013), pp. 6090-6093.

Biotransformation with whole microbial systems in a continuous flow reactor : resolution of (RS)-flurbiprofen using Aspergillus oryzae by direct esterification with ethanol in organic solvent

L. Tamborini
Primo
;
D. Romano
Secondo
;
A. Pinto;M. Contente;M.C. Iannuzzi;P. Conti
Penultimo
;
F. Molinari
Ultimo
2013

Abstract

Cell-bound lipases of dry mycelium of Aspergillus oryzae were used in organic solvent for the resolution of racemic flurbiprofen by direct esterification with ethanol in a flow-chemistry reactor. Under flow conditions a significant reduction of the reaction time and an increase of the enantioselectivity were achieved compared to the batch mode. Moreover, the process was implemented by adding an in-line purification step integrated with the racemization of the unreacted flurbiprofen directly into a polymer-supported resin.
Enzymatic resolution; Dry mycelia; Flow reactor; Flurbiprofen; Aspergillus oryzae
Settore CHIM/08 - Chimica Farmaceutica
Settore CHIM/11 - Chimica e Biotecnologia delle Fermentazioni
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/226269
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