Forty-two Lactobacillus helveticus strains isolated from whey starter cultures and cheeses were typed by multilocus restriction typing (MLRT), analyzing the restriction fragment-length polymorphism of PCR products generated from eight loci of housekeeping genes: b-galactosidase, lactose permease, UDP-galactose-4 epimerase, b-galactosidase transcriptional regulator, D-lactate dehydrogenase, peptidase N, stress-inducible protein and S-layer protein. MLRT analysis evidenced wide strain heterogeneity. Strains were grouped according to their source of isolation. The low infra-species polymorphism detected in the 16S rRNA gene did not enable to group the strains with the same sensitivity reached by MLRT of protein-coding genes. This is the first description of MLRT as a technique for the discrimination of L. helveticus strains; this technique seems a promising tool to evaluate genetic diversity of related strains from different ecological niches.
Multilocus restriction typing : a tool for studying molecular diversity within Lactobacillus helveticus of dairy origin / F. Borgo, G. Ricci, P.L. Manachini, M.G. Fortina. - In: INTERNATIONAL DAIRY JOURNAL. - ISSN 0958-6946. - 17:4(2007), pp. 336-342. [10.1016/j.idairyj.2006.03.002]
Multilocus restriction typing : a tool for studying molecular diversity within Lactobacillus helveticus of dairy origin
F. BorgoPrimo
;G. RicciSecondo
;P.L. ManachiniPenultimo
;M.G. Fortina
Ultimo
2007
Abstract
Forty-two Lactobacillus helveticus strains isolated from whey starter cultures and cheeses were typed by multilocus restriction typing (MLRT), analyzing the restriction fragment-length polymorphism of PCR products generated from eight loci of housekeeping genes: b-galactosidase, lactose permease, UDP-galactose-4 epimerase, b-galactosidase transcriptional regulator, D-lactate dehydrogenase, peptidase N, stress-inducible protein and S-layer protein. MLRT analysis evidenced wide strain heterogeneity. Strains were grouped according to their source of isolation. The low infra-species polymorphism detected in the 16S rRNA gene did not enable to group the strains with the same sensitivity reached by MLRT of protein-coding genes. This is the first description of MLRT as a technique for the discrimination of L. helveticus strains; this technique seems a promising tool to evaluate genetic diversity of related strains from different ecological niches.File | Dimensione | Formato | |
---|---|---|---|
int dairy j 2007.pdf
accesso riservato
Tipologia:
Publisher's version/PDF
Dimensione
277.96 kB
Formato
Adobe PDF
|
277.96 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.