Farm animals in intensive farming systems are frequently exposed to oxidative stress, which demands adequate antioxidant protection. The aim of this study was to evaluate the antioxidant potential of natural antioxidant mixture in case of n-6 PUFA induced oxidative stress in young pigs. Twenty four female piglets (Hypor) (BW 10 kg) were randomly assigned to three dietary treatments. Oxidative stress was induced in two of the three experimental groups with the inclusion of 9% of sunflower oil (SO) and the same inclusion of SO more a mixture of natural antioxidant (SO-NA). In control diet (CON) oil has been replaced with corn starch on the basis of net energy. Diets were isoenergetic by the adoption of appropriate quantity rationing differences. The animals were individually fed 2.7 times the maintenance requirements for metabolisable energy. From each piglets blood was collected by anterior vena cava puncture on days 0 (T0) and after 30 days (T1) of experimental period for the activity of Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GP) enzymes and assessment of total antiradicalic capacity by mean KRL test. KRL test measures the whole blood resistance to free radical attack and results were expressed as the time (min) required to reach 50% of haemolysis. Body weight and feed consumption were recorded weekly. No effect on growth performance was detected. Oxidative stress measured as KRL test showed a significantly reduction in both SO groups (P<0.001) and over time (P <0.001). The activity of enzymes did not differ among groups. Orthogonal contrast showed a significantly (P<0.001) activity of GPx reduction at T1 in groups treated with oil and a trend effect (P=0.081) of CAT enzyme. In conclusion dietary PUFA produced a reduction of antiradicalic capacity well highlighted by KRL test. The dosage of the enzyme activity of catalase, glutathione peroxidase and superoxide dismutase showed a pattern overlapping KRL test even if not always able to evidence significant difference among treatments. The natural mixture of antioxidants did not limit oxidative stress at level of the inclusion adopted.
Oxidative stress induced by high dietary PUFA in young pigs : effects on growth performance and some haematological parameters / G. Pastorelli, R. Rossi, C. Corino. - In: ITALIAN JOURNAL OF ANIMAL SCIENCE. - ISSN 1594-4077. - 12:Suppl. 1(2013), pp. C-077.33-C-077.33. ((Intervento presentato al 20. convegno ASPA Congress tenutosi a Bologna nel 2013.
|Titolo:||Oxidative stress induced by high dietary PUFA in young pigs : effects on growth performance and some haematological parameters|
PASTORELLI, GRAZIA (Primo)
ROSSI, RAFFAELLA (Secondo)
CORINO, CARLO (Ultimo)
|Settore Scientifico Disciplinare:||Settore AGR/18 - Nutrizione e Alimentazione Animale|
|Data di pubblicazione:||2013|
|Enti collegati al convegno:||Associazione scientifica di produzione animale|
|Appare nelle tipologie:||01 - Articolo su periodico|