To investigate the influence of diet on serum protein pattern, mice were fed for 8 weeks either control chow or a high-fat diet (containing 21 % w/w milk fat and 0.2 % w/w cholesterol); sera were collected and analyzed by 2-DE. The main positive acute-phase reactant proteins, haptoglobin and hemopexin, were significantly up-regulated in animals receiving the high-fat diet. Data on all other proteins also pointed to an inflammatory condition in these animals. The largest change in concentration was observed for carboxylesterase N, a circulating enzyme seldom connected with lipid metabolism in earlier reports. These observations agree with the notion of a link between diet-induced hyperlipidemia and the inflammatory component of its cardiovascular sequels in humans, but the effects in the experimental animals are massive and obviously affect most of the major serum proteins.
Inflammatory serum proteome pattern in mice fed a high-fat diet / E. Gianazza, C. Sensi, I. Eberini, F. Gilardi, M. Giudici, M. Crestani. - In: AMINO ACIDS. - ISSN 0939-4451. - 44:3(2013 Mar), pp. 1001-1008.
|Titolo:||Inflammatory serum proteome pattern in mice fed a high-fat diet|
|Parole Chiave:||Acute-phase reaction; Acute-phase reactant; Carboxylesterase; High-fat diet; Mouse; C-Reactive Protein; Immobilized Ph Gradients; Mesenchymal Stem-Cells; Acute-Phase Proteins; Apolipoprotein-A-I; E-Deficient Mice; Mouse Models; 2-Dimensional Electrophoresis; Rat Serum; Hypercholesterolemic Mice|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
|Data di pubblicazione:||mar-2013|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1007/s00726-012-1433-5|
|Appare nelle tipologie:||01 - Articolo su periodico|
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