A new HPLC/DAD (Diode Array Detector) method is proposed for the identification of some carotene isomers. The operating conditions adopted permit the resolution of alpha-carotene, all-trans-beta-carotene, 9-cis-beta-carotene, 13-cis-beta-carotene and 15-cis-beta-carotene. Moreover, the chromatographic conditions reported are simplified in respect of those reported up to now. The method is applied to the determination of carotenoids in a dried Dunaliella salina extract, but it could be also applied to other organic matrices such as eggs.

A simplified method for the HPLC resolution of alpha-carotene and beta-carotene (trans and cis) isomers / M. Bononi, I. Commissati, E. Lubian, A. Fossati, F. Tateo. - In: ANALYTICAL AND BIOANALYTICAL CHEMISTRY. - ISSN 1618-2642. - 372:(2002), pp. 401-403. [10.1007/s00216-001-1144-3]

A simplified method for the HPLC resolution of alpha-carotene and beta-carotene (trans and cis) isomers

M. Bononi
Primo
;
F. Tateo
Ultimo
2002

Abstract

A new HPLC/DAD (Diode Array Detector) method is proposed for the identification of some carotene isomers. The operating conditions adopted permit the resolution of alpha-carotene, all-trans-beta-carotene, 9-cis-beta-carotene, 13-cis-beta-carotene and 15-cis-beta-carotene. Moreover, the chromatographic conditions reported are simplified in respect of those reported up to now. The method is applied to the determination of carotenoids in a dried Dunaliella salina extract, but it could be also applied to other organic matrices such as eggs.
HPLC ; carotene isomers
Settore AGR/15 - Scienze e Tecnologie Alimentari
2002
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/212370
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