A comparative fluorescence in situ mapping of the SMN gene was performed on R-banded chromosome preparations of cattle (Bos taurus, BTA, 2n = 60), river buffalo (Bubalus bubalis, BBU, 2n = 50), sheep (Ovis aries, OAR, 2n = 54) and goat (Capra hircus, CHI, 2n = 60), as well as on those of a calf from Piedmont breed affected by arthrogryposis. SMN was located on BTA20q13.1, OAR16q13.1, CHI20q13.1 and BBU19q13. These chromosomes and chromosome bands are believed to be homeologous, confirming the high degree of chromosome homeologies among bovids. The position of SMN was refined in cattle, compared to the two previous localizations, while it is a new gene assignment in the other three bovids. A comparative fiber-FISH performed on extended chromatin of both normal cattle and calf affected by arthrogryposis revealed more extended FITC signals in the calf, compared to the normal cattle (control), suggesting a possible duplication of the SMN gene in the calf affected by arthrogryposis.
Comparative FISH-mapping of the survival of motor neuron gene (SMN) in domestic bovids / L. Iannuzzi, G.P. Di Meo, A. Perucatti, R. Rullo, D. Incarnato, M. Longeri, G. Bongioni, L. Molteni, A. Galli, M. Zanotti, A. Eggen. - In: CYTOGENETIC AND GENOME RESEARCH. - ISSN 1424-8581. - 102:1-4(2003 Sep), pp. 39-41. ((Intervento presentato al 4. convegno European Cytogenetics Conference tenutosi a Bologna nel 2003.
|Titolo:||Comparative FISH-mapping of the survival of motor neuron gene (SMN) in domestic bovids|
ZANOTTI, MARTA (Penultimo)
|Parole Chiave:||spinal muscular-atrophy; chromosome 20Q14; arthrogryposis; calves; maps|
|Settore Scientifico Disciplinare:||Settore AGR/17 - Zootecnica Generale e Miglioramento Genetico|
|Data di pubblicazione:||set-2003|
|Enti collegati al convegno:||European Cytogenetics Association|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1159/000075722|
|Appare nelle tipologie:||01 - Articolo su periodico|