The generation of human myogenic cell lines could potentially provide a valuable source for cell transplantation in myopathies. The dysregulation of proliferative-differentiative signals by viral oncogenes can result in the induction of apoptosis. Whether apoptosis occurred in myogenic cells expressing large T antigen (Tag) from SV40 upon differentiation was unknown. Human muscle satellite cells were transfected with two different constructs, containing either an origin-defective SV40 genome or Tag under vimentin promoter control. When differentiation was triggered, Tag expression reduced the formation of myotubes and dead cells showing apoptotic features were present. However, the cells expressing SV40 Tag under vimentin promoter control retained their capacity to form myotubes and expressed the myofibrillar proteins as myosin heavy chain and dystrophin when Tag expression was silent. Their apoptotic rate was similar to that of untransfected cells. The observation that apoptosis can be prevented by the down-regulation of Tag suggests that the programmed cell death induced in transformed cells can be reversed, and confirms the regulatory efficiency of the human vimentin promoter.

T-antigen regulated expression reduces apoptosis of tag-transformed human myoblasts / S.P. Corti, S. Salani, R. Del Bo, Y. Torrente, S. Strazzer, M. Belicchi, S. Paganoni, Z. Li, G.P. Comi, N. Bresolin, D. Paulin, G. Scarlato. - In: CELLULAR AND MOLECULAR LIFE SCIENCES. - ISSN 1420-682X. - 58:1(2001 Jan), pp. 135-140. [10.1007/PL00000773]

T-antigen regulated expression reduces apoptosis of tag-transformed human myoblasts

S.P. Corti
Primo
;
R. Del Bo;Y. Torrente;G.P. Comi;N. Bresolin;
2001

Abstract

The generation of human myogenic cell lines could potentially provide a valuable source for cell transplantation in myopathies. The dysregulation of proliferative-differentiative signals by viral oncogenes can result in the induction of apoptosis. Whether apoptosis occurred in myogenic cells expressing large T antigen (Tag) from SV40 upon differentiation was unknown. Human muscle satellite cells were transfected with two different constructs, containing either an origin-defective SV40 genome or Tag under vimentin promoter control. When differentiation was triggered, Tag expression reduced the formation of myotubes and dead cells showing apoptotic features were present. However, the cells expressing SV40 Tag under vimentin promoter control retained their capacity to form myotubes and expressed the myofibrillar proteins as myosin heavy chain and dystrophin when Tag expression was silent. Their apoptotic rate was similar to that of untransfected cells. The observation that apoptosis can be prevented by the down-regulation of Tag suggests that the programmed cell death induced in transformed cells can be reversed, and confirms the regulatory efficiency of the human vimentin promoter.
Clone Cells ; Apoptosis ; Humans ; Cell Differentiation ; Muscle Proteins ; Vimentin ; Muscle, Skeletal ; Promoter Regions, Genetic ; Down-Regulation ; Transfection ; Cells, Cultured ; Replication Origin ; Simian virus 40 ; Antigens, Polyomavirus Transforming ; Gene Expression Regulation ; Cell Line, Transformed ; Cell Transplantation ; DNA Fragmentation ; Immunohistochemistry ; Male
Settore MED/26 - Neurologia
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/207166
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