The Repressor Element 1 Silencing Transcription factor (REST/NRSF) is a master repressor of neuronal programs in non-neuronal lineages shown to function as a central regulator of developmental programs and stem cell physiology. Aberrant REST function has been associated with a number of pathological conditions. In cancer biology, REST has been shown to play a tumor suppressor activity in epithelial cancers but an oncogenic role in brain childhood malignancies such as neuroblastoma and medulloblastoma. Here we examined REST expression in human glioblastoma multiforme (GBM) specimens and its role in GBM cells carrying self-renewal and tumorigenic competence. We found REST to be expressed in GBM specimens, its presence being particularly enriched in tumor cells in the perivascular compartment. Significantly, REST is highly expressed in self-renewing tumorigenic-competent GBM cells and its knock down strongly reduces their self-renewal in vitro and tumor-initiating capacity in vivo and affects levels of miR-124 and its downstream targets. These results indicate that REST contributes to GBM maintenance by affecting its self-renewing and tumorigenic cellular component and that, hence, a better understanding of these circuitries in these cells might lead to new exploitable therapeutic targets.

REST Controls Self-Renewal and Tumorigenic Competence of Human Glioblastoma Cells / L. Conti, L. Crisafulli, V. Caldera, M. Tortoreto, E. Brilli, P. Conforti, F. Zunino, L. Magrassi, D. Schiffer, E. Cattaneo. - In: PLOS ONE. - ISSN 1932-6203. - 7:6(2012 Jun 11), pp. e38486.1-e38486.13.

REST Controls Self-Renewal and Tumorigenic Competence of Human Glioblastoma Cells

L. Conti;L. Crisafulli;P. Conforti;E. Cattaneo
2012

Abstract

The Repressor Element 1 Silencing Transcription factor (REST/NRSF) is a master repressor of neuronal programs in non-neuronal lineages shown to function as a central regulator of developmental programs and stem cell physiology. Aberrant REST function has been associated with a number of pathological conditions. In cancer biology, REST has been shown to play a tumor suppressor activity in epithelial cancers but an oncogenic role in brain childhood malignancies such as neuroblastoma and medulloblastoma. Here we examined REST expression in human glioblastoma multiforme (GBM) specimens and its role in GBM cells carrying self-renewal and tumorigenic competence. We found REST to be expressed in GBM specimens, its presence being particularly enriched in tumor cells in the perivascular compartment. Significantly, REST is highly expressed in self-renewing tumorigenic-competent GBM cells and its knock down strongly reduces their self-renewal in vitro and tumor-initiating capacity in vivo and affects levels of miR-124 and its downstream targets. These results indicate that REST contributes to GBM maintenance by affecting its self-renewing and tumorigenic cellular component and that, hence, a better understanding of these circuitries in these cells might lead to new exploitable therapeutic targets.
microRNA 124; protein REST; repressor element 1 silencing transcription factor; repressor protein; transcription factor; animal cell; animal experiment; animal model; article; carcinogenesis; cell compartmentalization; cell renewal; controlled study; downstream processing; glioblastoma; human; human cell; human tissue; in vitro study; in vivo study; mouse; nonhuman; protein expression; protein function; protein targeting; tumor cell
Settore BIO/14 - Farmacologia
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/206846
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